GeneTex
United States (US)

DPM1 antibody, N-term

GTX88562_915_IHC-P_18121411_341.jpg
GTX88562_2240_WB_18121411_194.jpg

Cat No. GTX88562

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88562-PEP).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence RELEVRSPRQNKYS-C, from the N Terminus of the protein sequence according to NP_003850.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dolichyl-Phosphate Mannosyltransferase Subunit 1, Catalytic,Cdgie,Mpds,Dpm1

Background

Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88562_915_IHC-P_18121411_341.jpg

GTX88562 IHC-P Image

IHC-P analysis of human tonsil using GTX88562 DPM1 antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX88562_2240_WB_18121411_194.jpg

GTX88562 WB Image

WB analysis of human liver lysate using GTX88562 DPM1 antibody, N-term.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329