GeneTex
United States (US)

DPM1 antibody [N1C2]

GTX106196_40562_20170615_WB_19011506_427.jpg

Cat No. GTX106196

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , SK-N-AS

Predict Reactivity

Mouse, Rat, Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DPM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CDGIE antibody, MPDS antibody, DPM1 antibody, DPM synthase antibody, dolichol monophosphate mannose synthase antibody, MPD synthase antibody, dolichyl-phosphate beta-D-mannosyltransferase antibody, dolichol-phosphate mannosyltransferase antibody, dolichol-phosphate mannose synthase antibody, mannose-P-dolichol synthase antibody, "dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit antibody"

Cellular Localization

Endoplasmic reticulum

Background

Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106196_40562_20170615_WB_19011506_427.jpg

GTX106196 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with DPM1 antibody [N1C2] (GTX106196) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169