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DPP3 antibody [N2C1], Internal

Anti-DPP3 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105949
Anti-DPP3 antibody [N2C1], Internal used in Western Blot (WB). GTX105949
Anti-DPP3 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX105949
Anti-DPP3 antibody [N2C1], Internal used in Western Blot (WB). GTX105949

Cat. No. GTX105949

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all DPP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

Molt-4 , BCL-1

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DPP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950139

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dipeptidyl peptidase 3 , DPPIII

Cellular Localization

Cytoplasm

Background

This gene encodes a protein that is a member of the S9B family in clan SC of the serine proteases. This cytoplasmic protein binds a single zinc ion with its zinc-binding motif (HELLGH) and has post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Increased activity of this protein is associated with endometrial and ovarian cancers. Alternate transcriptional splice variants have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DPP3 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105949

GTX105949 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Dipeptidyl-peptidase 3(GTX105949) antibody at 1:200 dilution.

Anti-DPP3 antibody [N2C1], Internal used in Western Blot (WB). GTX105949

GTX105949 WB Image

Sample(30 μg of whole cell lysate)
A:MOLT4(GTX27912)
10% SDS PAGE
GTX105949 diluted at 1:1000

Anti-DPP3 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX105949

GTX105949 IHC-P Image

DPP3 antibody [N2C1], Internal detects DPP3 protein at cytoplasm on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
DPP3 antibody [N2C1], Internal (GTX105949) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DPP3 antibody [N2C1], Internal used in Western Blot (WB). GTX105949

GTX105949 WB Image

DPP3 antibody [N2C1], Internal detects DPP3 protein by Western blot analysis.
A. 30 μg BCL-1 whole cell lysate/extract
7.5 % SDS-PAGE
DPP3 antibody [N2C1], Internal (GTX105949) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169