GeneTex
United States (US)

DPP3 antibody [N2C1], Internal

GTX105949_IFA_19011506_652.jpg
GTX105949_WB_19011506_570.jpg
GTX105949_39757_20141120_IHC_M_19011506_838.jpg
GTX105949_39757_WB_M_19011506_743.jpg

Cat No. GTX105949

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

Molt-4 , BCL-1

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.49mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DPP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DPPIII antibody, FLJ11387 antibody, FLJ22331 antibody, DPP3 antibody, dipeptidylpeptidase III antibody, dipeptidyl peptidase 3 antibody, dipeptidyl arylamidase III antibody, dipeptidyl peptidase III antibody, dipeptidylpeptidase 3 antibody, DPP III antibody, dipeptidyl aminopeptidase III antibody, dipeptidyl-peptidase 3 antibody

Cellular Localization

Cytoplasm

Background

This gene encodes a protein that is a member of the S9B family in clan SC of the serine proteases. This cytoplasmic protein binds a single zinc ion with its zinc-binding motif (HELLGH) and has post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Increased activity of this protein is associated with endometrial and ovarian cancers. Alternate transcriptional splice variants have been characterized. [provided by RefSeq]
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Database

DATA IMAGES
GTX105949_IFA_19011506_652.jpg

GTX105949 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Dipeptidyl-peptidase 3(GTX105949) antibody at 1:200 dilution.

GTX105949_WB_19011506_570.jpg

GTX105949 WB Image

Sample(30 μg of whole cell lysate)
A:MOLT4(GTX27912)
10% SDS PAGE
GTX105949 diluted at 1:1000

GTX105949_39757_20141120_IHC_M_19011506_838.jpg

GTX105949 IHC-P Image

DPP3 antibody [N2C1], Internal detects DPP3 protein at cytoplasm on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
DPP3 antibody [N2C1], Internal (GTX105949) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105949_39757_WB_M_19011506_743.jpg

GTX105949 WB Image

DPP3 antibody [N2C1], Internal detects DPP3 protein by Western blot analysis.
A. 30 µg BCL-1 whole cell lysate/extract
7.5 % SDS-PAGE
DPP3 antibody [N2C1], Internal (GTX105949) dilution: 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169