GeneTex
United States (US)

DR3 antibody

GTX105713_39974_WB_19011506_681.jpg
GTX105713_39974_IHC_19011506_181.jpg

Cat No. GTX105713

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

Raji , K562
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DR3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

APO-3 antibody, DDR3 antibody, DR3 antibody, LARD antibody, TNFRSF12 antibody, TR3 antibody, TRAMP antibody, WSL-1 antibody, WSL-LR antibody, TNFRSF25 antibody, apoptosis-mediating receptor TRAMP antibody, tumor necrosis factor receptor superfamily member 25 antibody, death receptor beta antibody, protein WSL-1 antibody, apoptosis inducing receptor antibody, tumor necrosis factor receptor superfamily, member 12 (translocating chain-association membrane protein) antibody, apoptosis-mediating receptor DR3 antibody, lymphocyte-associated receptor of death antibody, apoptosis-inducing receptor AIR antibody, death domain receptor 3 soluble form antibody, tumor necrosis factor receptor superfamily, member 25 antibody

Cellular Localization

Isoform 1: Cell membrane; Single-pass type I membrane protein , Isoform 2: Cell membrane; Single-pass type I membrane protein , Isoform 9: Cell membrane; Single-pass type I membrane protein , Isoform 11: Cell membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX105713_39974_WB_19011506_681.jpg

GTX105713 WB Image

DR3 antibody detects TNFRSF25 protein by Western blot analysis.
A. 30 µg Raji whole cell lysate/extract
B. 30 µg K562 whole cell lysate/extract
10 % SDS-PAGE
DR3 antibody (GTX105713) dilution: 1:1000

GTX105713_39974_IHC_19011506_181.jpg

GTX105713 IHC-P Image

DR3 antibody detects TNFRSF25 protein at cytosol on AGS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded AGS xenograft.
DR3 antibody (GTX105713) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf