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DR4 antibody

KO/KD
Anti-DR4 antibody used in IHC (Paraffin sections) (IHC-P). GTX28414
Anti-DR4 antibody used in Western Blot (WB). GTX28414
Anti-DR4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28414
Anti-DR4 antibody used in Western Blot (WB). GTX28414
Anti-DR4 antibody used in IHC (Paraffin sections) (IHC-P). GTX28414
Anti-DR4 antibody used in Western Blot (WB). GTX28414

Cat. No. GTX28414

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all DR4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ICC/IF 10 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

Jurkat Cell Lysate(GTX27899) , HepG2 Cell Lysate(GTX27900) , Human Spleen Tissue Slide

Product Note

Does not cross react with DR5.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids 427-445 of Human DR4.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_367920

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TNF receptor superfamily member 10a , APO2 , CD261 , DR4 , TRAILR-1 , TRAILR1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1and Fas. A novel cell death receptor was recently identified and designated DR4 (for death receptor4)(1). The ligand for this novel death receptor has been identified and termed TRAIL (2,3), which is a new member in the TNF family. DR4 is also called TRAIL receptor-1 (TRAIL-R1)(4). DR4 is expressed in most of human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4mediates apoptosis and NF-kB activation in many tissues and cells.

Database

  • Gene ID: 8797 TNFRSF10A
  • UniProt: O00220 TNFRSF10A

Research Area

DATA IMAGES

Anti-DR4 antibody used in IHC (Paraffin sections) (IHC-P). GTX28414

GTX28414 IHC-P Image

IHC-P analysis of human spleen tissue using GTX28414 DR4 antibody.
Working concentration : 20 μg/ml

Anti-DR4 antibody used in Western Blot (WB). GTX28414

GTX28414 WB Image

KO/KD

WB analysis of HeLa cell or DR4 KO cell lysates using GTX28414 DR4 antibody.
Dilution : 1μg/mL
Loading : 10μg

Anti-DR4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28414

GTX28414 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX28414 DR4 antibody.
Working concentration : 10 μg/ml

Anti-DR4 antibody used in Western Blot (WB). GTX28414

GTX28414 WB Image

WB analysis of various sample lysates using GTX28414 DR4 antibody.
Loading : 15μg of lysates per lane
Dilution : 1 μg/ml

Anti-DR4 antibody used in IHC (Paraffin sections) (IHC-P). GTX28414

GTX28414 IHC-P Image

IHC-P analysis of human spleen tissue using GTX28414 DR4 antibody.
Working concentration : 5 μg/ml

Anti-DR4 antibody used in Western Blot (WB). GTX28414

GTX28414 WB Image

WB analysis of (A) HepG2 and (B) Jurkat cell lysate using GTX28414 DR4 antibody.
Working concentration : 1 μg/ml

REFERENCE

Application Reference

Hayashi Y et al. PLoS One 2021; 16 (11) : e0260443 Virus-infection in cochlear supporting cells induces audiosensory receptor hair cell death by TRAIL-induced necroptosis.
Application : IHC-P
Reactivity : Mouse
Greer YE et al. Breast Cancer Res 2019; 21 (1) : 27 MEDI3039, a novel highly potent tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) receptor 2 agonist, causes regression of orthotopic tumors and inhibits outgrowth of metastatic triple-negative breast cancer.
Dine JL et al. Breast Cancer Res Treat 2016; 155 (2) : 235-51 The TRAIL receptor agonist drozitumab targets basal B triple-negative breast cancer cells that express vimentin and Axl.
Application : WB
Reactivity : Human
Ou YC et al. Life Sci 2014; 100 (2) : 110-7 Luteolin sensitizes human 786-O renal cell carcinoma cells to TRAIL-induced apoptosis.
Application : WB
Reactivity : Human
Garimella SV et al. Mol Cancer Res 2012; 10 (1) : 75-85 WEE1 inhibition sensitizes basal breast cancer cells to TRAIL-induced apoptosis.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399