GeneTex
United States (US)

DR4 antibody

GTX28414_70_ICC-IF_20180221_18121410_683.jpg
GTX28414_2448_WB_20180221_18121410_327.jpg
GTX28414_852_IHC-P_20180221_18121410_207.jpg
GTX28414_853_IHC-P_20180221_18121410_295.jpg

Cat No. GTX28414

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
ICC/IF 10 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

Jurkat Cell Lysate(GTX27899) , HepG2 Cell Lysate(GTX27900) , Human Spleen Tissue Slide

Specificity/Sensitivity

Does not cross react with DR5.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids 427-445 of Human DR4.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tnf Receptor Superfamily Member 10A,Apo2,Cd261,Dr4,Trailr-1,Trailr1,Tnfrsf10A

Cellular Localization

Cell Membrane

Background

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1and Fas. A novel cell death receptor was recently identified and designated DR4 (for death receptor4)(1). The ligand for this novel death receptor has been identified and termed TRAIL (2,3), which is a new member in the TNF family. DR4 is also called TRAIL receptor-1 (TRAIL-R1)(4). DR4 is expressed in most of human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4mediates apoptosis and NF-kB activation in many tissues and cells.

Database

Research Area

DATA IMAGES
GTX28414_70_ICC-IF_20180221_18121410_683.jpg

GTX28414 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX28414 DR4 antibody.
Working concentration : 10 μg/ml

GTX28414_2448_WB_20180221_18121410_327.jpg

GTX28414 WB Image

WB analysis of (A) HepG2 and (B) Jurkat cell lysate using GTX28414 DR4 antibody.
Working concentration : 1 μg/ml

GTX28414_852_IHC-P_20180221_18121410_207.jpg

GTX28414 IHC-P Image

IHC-P analysis of human spleen tissue using GTX28414 DR4 antibody.
Working concentration : 5 μg/ml

GTX28414_853_IHC-P_20180221_18121410_295.jpg

GTX28414 IHC-P Image

IHC-P analysis of human spleen tissue using GTX28414 DR4 antibody.
Working concentration : 20 μg/ml

REFERENCE
Package List Price ($)
$ 339