GeneTex
United States (US)

DRAK2 antibody

DRAK2-antibody_WB_GTX28419-1_18121410_432.jpg
DRAK2-antibody_ICCIF_GTX28419-1_18121410_915.jpg

Cat No. GTX28419

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

Western blot at 1:500 to 1:2000 dilution.

Calculated MW

42 kDa. ( Note )

Positive Control

Wholecell lysate from Jurkat cells An approximately 45 kDa band can be detected.

Specificity/Sensitivity

None to DAP or ZIP kinases. The approximately 70 kDa band visible on WesGTX11950tern blots is probably non-related to DRAK2 although it is peptide blockable with GTX28454.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide, corresponding to amino acids 351-365 of Human DRAK2.

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Nuclear

Background

Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 (for DAP kinase-related apoptosis-inducing protein kinases) (1). DRAKs contain an N-terminal kinase domain and a C-terminal regulation domain. Overexpression of DRAK2 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. DRAK2 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues (1).

Database

Research Area

DATA IMAGES
DRAK2-antibody_WB_GTX28419-1_18121410_432.jpg

GTX28419 WB Image

Western blot analysis of DRAK2 in Jurkat (1,3) and Raji (2,4) cell lysate in the absence (1,2) or presence (3,4) of blocking peptide with DRAK2 antibody (GTX28419) at 1 g/ml.

DRAK2-antibody_ICCIF_GTX28419-1_18121410_915.jpg

GTX28419 ICC/IF Image

Immunocytochemical staining of DRAK2 in Jurkat cells with DRAK2 antibody (GTX28419) at 10μg/ml.

Package List Price ($)
$ 329