Western blot at 1:500 to 1:2000 dilution.
Wholecell lysate from Jurkat cells An approximately 45 kDa band can be detected.
None to DAP or ZIP kinases. The approximately 70 kDa band visible on WesGTX11950tern blots is probably non-related to DRAK2 although it is peptide blockable with GTX28454.
PBS with 0.02% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
0.5mg/ml(Please refer to the vial label for the specific concentration.)
Synthetic peptide, corresponding to amino acids 351-365 of Human DRAK2.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 (for DAP kinase-related apoptosis-inducing protein kinases) (1). DRAKs contain an N-terminal kinase domain and a C-terminal regulation domain. Overexpression of DRAK2 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. DRAK2 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues (1).