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DRAM antibody

Anti-DRAM antibody used in Western Blot (WB). GTX85484
Anti-DRAM antibody used in IHC (Paraffin sections) (IHC-P). GTX85484
Anti-DRAM antibody used in IHC (Paraffin sections) (IHC-P). GTX85484

Cat. No. GTX85484

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all DRAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

HEK293 Cell Lysate(GTX27902) , Human Liver Tissue Slide , Human Liver Tissue Lysate

Product Note

This antibody is specific for DRAM C-Terminus

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

DRAM antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human DRAM.The immunogen is located within amino acids 170 - 220 of DRAM.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA damage regulated autophagy modulator 1 , DRAM

Cellular Localization

Lysosome membrane

Background

Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and suggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.

Database

Research Area

DATA IMAGES

Anti-DRAM antibody used in Western Blot (WB). GTX85484

GTX85484 WB Image

WB analysis of 293 cell lysate using GTX85484 DRAM antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

Anti-DRAM antibody used in IHC (Paraffin sections) (IHC-P). GTX85484

GTX85484 IHC-P Image

IHC-P analysis of human liver tissue using GTX85484 DRAM antibody.
Working concentration : 2.5 μg/ml

Anti-DRAM antibody used in IHC (Paraffin sections) (IHC-P). GTX85484

GTX85484 IHC-P Image

IHC-P analysis of human liver tissue using GTX85484 DRAM antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399