GeneTex
United States (US)

DRIL1 antibody [N2C2], Internal

GTX105720_39974_IP_19011506_207.jpg
GTX105720_39974_IFA_19011506_821.jpg
GTX105720_IHC_19011506_362.jpg
GTX105720_39974_WB_M_19011506_482.jpg
GTX105720_39974_WB_19011506_475.jpg

Cat No. GTX105720

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

HepG2 , BCL-1

Predict Reactivity

Rat, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DRIL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BRIGHT antibody, DRIL1 antibody, DRIL3 antibody, E2FBP1 antibody, ARID3A antibody, ARID domain-containing 3A antibody, AT rich interactive domain 3A (BRIGHT- like) protein antibody, dead ringer-like 1 antibody, dead ringer-like protein 1 antibody, AT-rich interactive domain-containing protein 3A antibody, B-cell regulator of IgH transcription antibody, E2F-binding protein 1 antibody, ARID domain-containing protein 3A antibody, AT rich interactive domain 3A (BRIGHT-like) antibody

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a member of the ARID (AT-rich interaction domain) family of DNA binding proteins. It was found by homology to the Drosophila dead ringer gene, which is important for normal embryogenesis. Other ARID family members have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation, and possibly in chromatin structure modification. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105720_39974_IP_19011506_207.jpg

GTX105720 IP Image

DRIL1 antibody immunoprecipitates DRIL1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of DRIL1 protein by 2 μg of DRIL1 antibody (GTX105720) 7.5% SDS-PAGE The immunoprecipitated DRIL1 protein was detected by DRIL1 antibody (GTX105720) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX105720_39974_IFA_19011506_821.jpg

GTX105720 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using DRIL1(GTX105720) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX105720_IHC_19011506_362.jpg

GTX105720 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using ARID3A(GTX105720) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105720_39974_WB_M_19011506_482.jpg

GTX105720 WB Image

DRIL1 antibody [N2C2], Internal detects ARID3A protein by Western blot analysis.
A. 30 µg BCL-1 whole cell lysate/extract
7.5 % SDS-PAGE
DRIL1 antibody [N2C2], Internal (GTX105720) dilution: 1:1000

GTX105720_39974_WB_19011506_475.jpg

GTX105720 WB Image

DRIL1 antibody [N2C2], Internal detects ARID3A protein by Western blot analysis.
A. 30 µg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
DRIL1 antibody [N2C2], Internal (GTX105720) dilution: 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169