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DRIL1 antibody

Orthogonal
Anti-DRIL1 antibody used in Immunoprecipitation (IP). GTX114367
Anti-DRIL1 antibody used in Western Blot (WB). GTX114367
Anti-DRIL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114367
Anti-DRIL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114367
Anti-DRIL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114367

Cat. No. GTX114367

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all DRIL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

HCT116

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DRIL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619932

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AT-rich interaction domain 3A , BRIGHT , DRIL1 , DRIL3 , E2FBP1

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a member of the ARID (AT-rich interaction domain) family of DNA binding proteins. It was found by homology to the Drosophila dead ringer gene, which is important for normal embryogenesis. Other ARID family members have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation, and possibly in chromatin structure modification. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DRIL1 antibody used in Immunoprecipitation (IP). GTX114367

GTX114367 IP Image

DRIL1 antibody immunoprecipitates DRIL1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of DRIL1 protein by 2 μg of DRIL1 antibody (GTX114367) 7.5% SDS-PAGE The immunoprecipitated DRIL1 protein was detected by DRIL1 antibody (GTX114367) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-DRIL1 antibody used in Western Blot (WB). GTX114367

GTX114367 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DRIL1 antibody (GTX114367) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-DRIL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114367

GTX114367 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DRIL1(GTX114367) antibody at 1:500 dilution.

Anti-DRIL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114367

GTX114367 IHC-P Image

DRIL1 antibody detects DRIL1 protein at cytosol and nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
DRIL1 antibody (GTX114367) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DRIL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114367

GTX114367 IHC-P Image

DRIL1 antibody detects DRIL1 protein at cytosol and nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
DRIL1 antibody (GTX114367) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169