DSN1 antibody [N3C3]

Name: DSN1 antibody [N3C3]
Brand: GeneTex
SKU: GTX120402
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-DSN1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120402

Anti-DSN1 antibody [N3C3] used in Western Blot (WB). GTX120402

Cat. No. GTX120402

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all DSN1 products

View product citations for antibody GTX120402 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000

Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DSN1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721038

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DSN1 component of MIS12 kinetochore complex , C20orf172 , KNL3 , MIS13 , dJ469A13.2 , hKNL-3

Background

This gene encodes a kinetochore protein that functions as part of the minichromosome instability-12 centromere complex. The encoded protein is required for proper kinetochore assembly and progression through the cell cycle. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Gene ID: 79980 DSN1
UniProt: Q9H410 DSN1

Research Area

DATA IMAGES

GTX120402 ICC/IF Image

HeLa cells were fixed with 2% PFA for IF staining. Primary Ab diluted at 1: 1000 with 10% normal donkey serum in PHEM buffer.
ACA is used as a marker for inner kinetochore.
(Image kindly provided by Drs. J. Herman and J. DeLuca, Colorado State University)

GTX120402 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with DSN1 antibody [N3C3] (GTX120402) diluted at 1:500.

REFERENCE

Application Reference

Hadders MA et al. J Cell Biol 2020; 219 (3) Untangling the contribution of Haspin and Bub1 to Aurora B function during mitosis.

Burke J.M. et al. bioRxiv 2017; (Epub) The Ndc80 complex targets Bod1 to human mitotic kinetochores.

Application : ICC/IF

Reactivity : Human

Milev MP et al. J Cell Biol 2015; 209 (2) : 221-34 TRAMM/TrappC12 plays a role in chromosome congression, kinetochore stability, and CENP-E recruitment.

Caldas GV et al. J Cell Biol 2013; 203 (6) : 957-69 KNL1 facilitates phosphorylation of outer kinetochore proteins by promoting Aurora B kinase activity.

Application : ICC/IF

Reactivity : Human

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