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DSN1 antibody

KO/KD
Anti-DSN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00889
Anti-DSN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00889
Anti-DSN1 antibody used in Western Blot (WB). GTX00889
Anti-DSN1 antibody used in Western Blot (WB). GTX00889

Cat. No. GTX00889

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all DSN1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant GST-DSN1 (1-356 aa)

Purification

Purified IgG
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DSN1 component of MIS12 kinetochore complex , C20orf172 , DSN1 , DSN1 homolog, MIS12 kinetochore complex component , KNL3 , MIS13 , dJ469A13.2 , hKNL3

Cellular Localization

Nucleus,Chromosome,Centromere,Kinetochore

Background

This gene encodes a kinetochore protein that functions as part of the minichromosome instability-12 centromere complex. The encoded protein is required for proper kinetochore assembly and progression through the cell cycle. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2009]

Database

Research Area

DATA IMAGES

Anti-DSN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00889

GTX00889 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00889 DSN1 antibody. DSN1 is abundantly expressed in mitotic cells on mitotic apparatus.
Fixation : 4% PFA
Dilution : 1:100

Anti-DSN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00889

GTX00889 ICC/IF Image

KO/KD

ICC/IF analysis of HeLa cells using GTX00889 DSN1 antibody. Mitotic cells were accumulated by nocodazole treatment and chromosomes were spread on coverglass.
Left : Cells transfected with luciferase siRNA (as a control)
Right : Cells transfected with DSN1 siRNA

Anti-DSN1 antibody used in Western Blot (WB). GTX00889

GTX00889 WB Image

WB analysis of MCF-7 whole cell lysate using GTX00889 DSN1 antibody.
Dilution : 1:1000
Loading : 20 μg

Anti-DSN1 antibody used in Western Blot (WB). GTX00889

GTX00889 WB Image

KO/KD

WB analysis of HeLa whole cell lysate using GTX00889 DSN1 antibody.
Lane 1 : Cells transfected with luciferase siRNA (as a control)
Lane 2 : Cells transfected with DSN1 siRNA
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399