APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000 |
1:1000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Serum
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant GST-DSN1 (1-356 aa)
Purification
Purified IgG
From serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DSN1 component of MIS12 kinetochore complex , C20orf172 , DSN1 , DSN1 homolog, MIS12 kinetochore complex component , KNL3 , MIS13 , dJ469A13.2 , hKNL3
Cellular Localization
Nucleus,Chromosome,Centromere,Kinetochore
Background
This gene encodes a kinetochore protein that functions as part of the minichromosome instability-12 centromere complex. The encoded protein is required for proper kinetochore assembly and progression through the cell cycle. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2009]
Database
Research Area
DATA IMAGES
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GTX00889 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX00889 DSN1 antibody. DSN1 is abundantly expressed in mitotic cells on mitotic apparatus. Fixation : 4% PFA Dilution : 1:100
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GTX00889 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX00889 DSN1 antibody. Mitotic cells were accumulated by nocodazole treatment and chromosomes were spread on coverglass. Left : Cells transfected with luciferase siRNA (as a control) Right : Cells transfected with DSN1 siRNA
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GTX00889 WB Image
WB analysis of MCF-7 whole cell lysate using GTX00889 DSN1 antibody. Dilution : 1:1000 Loading : 20 μg
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GTX00889 WB Image
WB analysis of HeLa whole cell lysate using GTX00889 DSN1 antibody. Lane 1 : Cells transfected with luciferase siRNA (as a control) Lane 2 : Cells transfected with DSN1 siRNA Dilution : 1:1000
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REFERENCE
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REVIEW
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