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DUOX1 antibody [N1N3]

Anti-DUOX1 antibody [N1N3] used in Western Blot (WB). GTX119160
Anti-DUOX1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX119160
Anti-DUOX1 antibody [N1N3] used in Western Blot (WB). GTX119160

Cat No. GTX119160

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

177 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , unboiled A431 , A431 membrane extract

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DUOX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617133

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

dual oxidase 1 , LNOX1 , NOXEF1 , THOX1

Cellular Localization

Apical cell membrane

Background

The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes this encoded protein and DUOX2. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DUOX1 antibody [N1N3] used in Western Blot (WB). GTX119160

GTX119160 WB Image

Boiled and unboiled A431 whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DUOX1 antibody [N1N3] (GTX119160) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Anti-DUOX1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX119160

GTX119160 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using DUOX1(GTX119160) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DUOX1 antibody [N1N3] used in Western Blot (WB). GTX119160

GTX119160 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DUOX1 antibody [N1N3] (GTX119160) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

DUOX1 antibody [N1N3] Cat No. GTX119160

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017. PMID: 28099519
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169