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DUS2L antibody

Anti-DUS2L antibody used in Western Blot (WB). GTX121407
Anti-DUS2L antibody used in IHC (Paraffin sections) (IHC-P). GTX121407

Cat. No. GTX121407

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DUS2L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DUS2L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164894

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dihydrouridine synthase 2 , DUS2L , SMM1 , URLC8

Cellular Localization

Cytoplasm , Endoplasmic reticulum

Background

Dihydrouridine synthase catalyzes reduction of the 5,6-double bond of a uridine residue on the displacement loop of tRNA. The resultant modified base, 5,6-dihydrouridine, appears to increase the conformational flexibility and dynamic motion of tRNA (Kato et al., 2005 [PubMed 15994936]).[supplied by OMIM]

Database

DATA IMAGES

Anti-DUS2L antibody used in Western Blot (WB). GTX121407

GTX121407 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
B: HepG2
10% SDS PAGE
GTX121407 diluted at 1:1000

Anti-DUS2L antibody used in IHC (Paraffin sections) (IHC-P). GTX121407

GTX121407 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using DUS2L(GTX121407) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169