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DUSP1 (phospho Ser296/Ser318) antibody

Anti-DUSP1 (phospho Ser296/Ser318) antibody used in Western Blot (WB). GTX52320

Cat No. GTX52320

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC

Reactivity

Human, Mouse, Rat
Package
100 μg ($319)

APPLICATION

Application Note

WB 1:500-1:2000, IHC 1:50-1:200

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human DUSP1 around the phosphorylation site of Ser296/Ser318

Purification

Antigen affinity purification

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Dual Specificity Phosphatase 1 , Cl100 , Hvh1 , Mkp-1 , Mkp1 , Ptpn10 , Dusp1

Background

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-DUSP1 (phospho Ser296/Ser318) antibody used in Western Blot (WB). GTX52320

GTX52320 WB Image

Western blot analysis of MKP-1/2 phosphorylation expression in heatshockK562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf