*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
A549 , HeLa , HepG2 , HCT116
Mouse, Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Recombinant protein encompassing a sequence within the center region of human DUSP10. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
MKP-5 antibody, MKP5 antibody, DUSP10 antibody, map kinase phosphatase 5 antibody, serine/threonine specific protein phosphatase antibody, mitogen-activated protein kinase phosphatase 5 antibody, dual specificity protein phosphatase 10 antibody, dual specificity phosphatase MKP-5 antibody, dual specificity phosphatase 10 antibody
Cytoplasm , Nucleus
Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK, but not MAPK/ERK. Its subcellular localization is unique; it is evenly distributed in both the cytoplasm and the nucleus. This gene is widely expressed in various tissues and organs, and its expression is elevated by stress stimuli. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]
GTX115564 IHC-P Image
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using DUSP10(GTX115564) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX115564 WB Image
Sample (30 ug of whole cell lysate)
10% SDS PAGE
GTX115564 diluted at 1:1000
GTX115564 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DUSP10(GTX115564) antibody at 1:200 dilution.