GeneTex
United States (US)

DUSP10 antibody [N1C1]

GTX115564_40184_IHC_18121410_132.jpg
GTX115564_WB_18121410_404.jpg
GTX115564_40184_IFA_18121410_206.jpg

Cat No. GTX115564

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DUSP10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MKP-5 antibody, MKP5 antibody, DUSP10 antibody, map kinase phosphatase 5 antibody, serine/threonine specific protein phosphatase antibody, mitogen-activated protein kinase phosphatase 5 antibody, dual specificity protein phosphatase 10 antibody, dual specificity phosphatase MKP-5 antibody, dual specificity phosphatase 10 antibody

Cellular Localization

Cytoplasm , Nucleus

Background

Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK, but not MAPK/ERK. Its subcellular localization is unique; it is evenly distributed in both the cytoplasm and the nucleus. This gene is widely expressed in various tissues and organs, and its expression is elevated by stress stimuli. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]
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Database

DATA IMAGES
GTX115564_40184_IHC_18121410_132.jpg

GTX115564 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using DUSP10(GTX115564) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115564_WB_18121410_404.jpg

GTX115564 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: HCT116
10% SDS PAGE
GTX115564 diluted at 1:1000

GTX115564_40184_IFA_18121410_206.jpg

GTX115564 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DUSP10(GTX115564) antibody at 1:200 dilution.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169