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DUSP3 antibody [N1C3]

Anti-DUSP3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109149
Anti-DUSP3 antibody [N1C3] used in Western Blot (WB). GTX109149
Anti-DUSP3 antibody [N1C3] used in Western Blot (WB). GTX109149

Cat. No. GTX109149

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all DUSP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

Mouse stomach , 293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human DUSP3 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950151

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dual specificity phosphatase 3 , VHR

Background

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. [provided by RefSeq]

Database

DATA IMAGES

Anti-DUSP3 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109149

GTX109149 IHC-P Image

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using DUSP3(GTX109149) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DUSP3 antibody [N1C3] used in Western Blot (WB). GTX109149

GTX109149 WB Image

DUSP3 antibody detects DUSP3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15 % SDS-PAGE, and blotted with DUSP3 antibody (GTX109149) diluted by 1:1000

Anti-DUSP3 antibody [N1C3] used in Western Blot (WB). GTX109149

GTX109149 WB Image

Sample (50 ug of whole cell lysate)
A: mouse stomach
12% SDS PAGE
GTX109149 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169