APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
A549
Predict Reactivity
Mouse, Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.91 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human DUSP6. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10624666
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
dual specificity phosphatase 6 , HH19 , MKP3 , PYST1
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-DUSP6 antibody [N3C3] used in Western Blot (WB). GTX104449](/templates/default/js/lightbox/images/loading.gif "Anti-DUSP6 antibody [N3C3] used in Western Blot (WB). GTX104449")
|
GTX104449 WB Image
Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX104449 diluted at 1:1000
|
REFERENCE
There are currently no references for DUSP6 antibody [N3C3] (GTX104449). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for DUSP6 antibody [N3C3] (GTX104449). Be the first to share your experience with this product.
![Anti-DUSP6 antibody [N3C3] used in Western Blot (WB). GTX104449](/upload/website/prouct_img/small/GTX104449/GTX104449_WB_w_23060120_717.webp "Anti-DUSP6 antibody [N3C3] used in Western Blot (WB). GTX104449")
![Anti-DUSP6 antibody [N3C3] used in Western Blot (WB). GTX104449](/upload/website/prouct_img/normal/GTX104449/GTX104449_WB_w_23060120_717.webp "Anti-DUSP6 antibody [N3C3] used in Western Blot (WB). GTX104449")