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DUSP8 antibody [HL2338]

Anti-DUSP8 antibody [HL2338] used in Western Blot (WB). GTX638541
Anti-DUSP8 antibody [HL2338] used in Western Blot (WB). GTX638541
Anti-DUSP8 antibody [HL2338] used in Western Blot (WB). GTX638541
Anti-DUSP8 antibody [HL2338] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638541
Anti-DUSP8 antibody [HL2338] used in Western Blot (WB). GTX638541
Anti-DUSP8 antibody [HL2338] used in IHC (Paraffin sections) (IHC-P). GTX638541
Anti-DUSP8 antibody [HL2338] used in Western Blot (WB). GTX638541

Cat. No. GTX638541

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2338

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human DUSP8

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dual specificity phosphatase 8 , C11orf81 , HB5 , HVH-5 , HVH8

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin. An intronless pseudogene for DUSP8 is present on chromosome 10q11.2. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-DUSP8 antibody [HL2338] used in Western Blot (WB). GTX638541

GTX638541 WB Image

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DUSP8 antibody [HL2338] (GTX638541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DUSP8 antibody [HL2338] used in Western Blot (WB). GTX638541

GTX638541 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DUSP8 antibody [HL2338] (GTX638541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DUSP8 antibody [HL2338] used in Western Blot (WB). GTX638541

GTX638541 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DUSP8 antibody [HL2338] (GTX638541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DUSP8 antibody [HL2338] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638541

GTX638541 ICC/IF Image

DUSP8 antibody [HL2338] detects DUSP8 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DUSP8 stained by DUSP8 antibody [HL2338] (GTX638541) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-DUSP8 antibody [HL2338] used in Western Blot (WB). GTX638541

GTX638541 WB Image

Human tissue extract (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DUSP8 antibody [HL2338] (GTX638541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DUSP8 antibody [HL2338] used in IHC (Paraffin sections) (IHC-P). GTX638541

GTX638541 IHC-P Image

DUSP8 antibody [HL2338] detects DUSP8 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
DUSP8 stained by DUSP8 antibody [HL2338] (GTX638541) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DUSP8 antibody [HL2338] used in Western Blot (WB). GTX638541

GTX638541 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DUSP8 antibody [HL2338] (GTX638541) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations