APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89940-PEP).
Product Note
This antibody is also expected to recognise the hypothetical human protein similar to dual specificity phosphatase 8 (XM_114902), which is virtually identical.
Predict Reactivity
Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence AGDRLPRKVMDAK-C, from the N Terminus of the protein sequence according to NP_004411.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
dual specificity phosphatase 8 , C11orf81 , HB5 , HVH-5 , HVH8
Cellular Localization
Cytoplasm , Nucleus
Background
The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin. An intronless pseudogene for DUSP8 is present on chromosome 10q11.2. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX89940 WB Image
WB analysis of human cerebellum lysate using GTX89940 DUSP8 antibody, N-term.
Dilution : 2μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for DUSP8 antibody, N-term (GTX89940). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for DUSP8 antibody, N-term (GTX89940). Be the first to share your experience with this product.
