GeneTex
United States (US)

DUSP8 antibody

GTX115561_41024_IFA_18121410_634.jpg
GTX115561_41024_WB_18121410_191.jpg

Cat No. GTX115561

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

Jurkat , Raji

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DUSP8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dual Specificity Phosphatase 8,C11Orf81,Hb5,Hvh-5,Hvh8,Dusp8

Background

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin. An intronless pseudogene for DUSP8 is present on chromosome 10q11.2. [provided by RefSeq]

Database

DATA IMAGES
GTX115561_41024_IFA_18121410_634.jpg

GTX115561 ICC/IF Image

DUSP8 antibody detects DUSP8 protein at cytoplasm by immunofluorescent analysis.
Sample: RD cells were fixed in ice-cold MeOH for 5 min.
Green: DUSP8 protein stained by DUSP8 antibody (GTX115561) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX115561_41024_WB_18121410_191.jpg

GTX115561 WB Image

DUSP8 antibody detects DUSP8 protein by western blot analysis.
A. 30 µg Jurkat whole cell extract
B. 30 µg Raji whole cell extract
7.5% SDS-PAGE
DUSP8 antibody (GTX115561) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169