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DYNC1I2 antibody

Anti-DYNC1I2 antibody used in IHC (Paraffin sections) (IHC-P). GTX112419
Anti-DYNC1I2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112419
Anti-DYNC1I2 antibody used in Western Blot (WB). GTX112419
Anti-DYNC1I2 antibody used in IHC (Paraffin sections) (IHC-P). GTX112419

Cat. No. GTX112419

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($419),
25 μl ($169)
See all DYNC1I2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DYNC1I2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036810

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dynein cytoplasmic 1 intermediate chain 2 , DIC74 , DNCI2 , IC2

Background

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes.

Database

Research Area

DATA IMAGES

Anti-DYNC1I2 antibody used in IHC (Paraffin sections) (IHC-P). GTX112419

GTX112419 IHC-P Image

DYNC1I2 antibody detects DYNC1I2 protein at cytosol on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
DYNC1I2 antibody (GTX112419) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DYNC1I2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112419

GTX112419 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using DYNC1I2(GTX112419) antibody at 1:200 dilution.

Anti-DYNC1I2 antibody used in Western Blot (WB). GTX112419

GTX112419 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX112419 diluted at 1:1000

Anti-DYNC1I2 antibody used in IHC (Paraffin sections) (IHC-P). GTX112419

GTX112419 IHC-P Image

DYNC1I2 antibody detects DYNC1I2 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
DYNC1I2 antibody (GTX112419) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169