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DYNC1I2 antibody

Anti-DYNC1I2 antibody used in IHC (Paraffin sections) (IHC-P). GTX112419
Anti-DYNC1I2 antibody used in Western Blot (WB). GTX112419
Anti-DYNC1I2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112419
Anti-DYNC1I2 antibody used in IHC (Paraffin sections) (IHC-P). GTX112419

Cat No. GTX112419

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DYNC1I2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036810

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

dynein cytoplasmic 1 intermediate chain 2 , DIC74 , DNCI2 , IC2

Background

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes.

Database

Research Area

DATA IMAGES

Anti-DYNC1I2 antibody used in IHC (Paraffin sections) (IHC-P). GTX112419

GTX112419 IHC-P Image

DYNC1I2 antibody detects DYNC1I2 protein at cytosol on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
DYNC1I2 antibody (GTX112419) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DYNC1I2 antibody used in Western Blot (WB). GTX112419

GTX112419 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX112419 diluted at 1:1000

Anti-DYNC1I2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112419

GTX112419 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using DYNC1I2(GTX112419) antibody at 1:200 dilution.

Anti-DYNC1I2 antibody used in IHC (Paraffin sections) (IHC-P). GTX112419

GTX112419 IHC-P Image

DYNC1I2 antibody detects DYNC1I2 protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
DYNC1I2 antibody (GTX112419) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for DYNC1I2 antibody (GTX112419). Be the first to share your publications with this product.

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169