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DYNC1LI1 antibody

Anti-DYNC1LI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX120114
Anti-DYNC1LI1 antibody used in Western Blot (WB). GTX120114
Anti-DYNC1LI1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120114
Anti-DYNC1LI1 antibody used in Western Blot (WB). GTX120114
Anti-DYNC1LI1 antibody used in Immunoprecipitation (IP). GTX120114

Cat. No. GTX120114

Reference 8
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all DYNC1LI1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

293T , Mouse brain

Predict Reactivity

Bovine, Chicken, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DYNC1LI1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10720534

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dynein cytoplasmic 1 light intermediate chain 1 , DLC-A , DNCLI1 , LIC1

Background

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes. Probably involved in the microtubule-dependent transport of pericentrin. Is required for progress throuh the spindle assembly checkpoint. The phosphorylated form appears to be involved in the selective removal of MAD1L1 and MAD1L2 but not BUB1B from kinetochores.

Database

Research Area

DATA IMAGES

Anti-DYNC1LI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX120114

GTX120114 IHC-P Image

DYNC1LI1 antibody detects DYNC1LI1 protein at cytoplasm in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
DYNC1LI1 antibody (GTX120114) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DYNC1LI1 antibody used in Western Blot (WB). GTX120114

GTX120114 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX120114 diluted at 1:1000

Anti-DYNC1LI1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120114

GTX120114 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using DYNC1LI1(GTX120114) antibody at 1:500 dilution.

Anti-DYNC1LI1 antibody used in Western Blot (WB). GTX120114

GTX120114 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX120114 diluted at 1:5000

Anti-DYNC1LI1 antibody used in Immunoprecipitation (IP). GTX120114

GTX120114 IP Image

Immunoprecipitation of DYNC1LI1 protein from 293T whole cell extracts using 5 μg of DYNC1LI1 antibody (GTX120114).
Western blot analysis was performed using DYNC1LI1 antibody (GTX120114).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

Application Reference

Y Wu et al. Metabolites 2024; 14 (1) : 51 Integrating TCGA and Single-Cell Sequencing Data for Hepatocellular Carcinoma: A Novel Glycosylation (GLY)/Tumor Microenvironment (TME) Classifier to Predict Prognosis and Immunotherapy Response
Application : WB
Reactivity : Human
Eibes S et al. Nat Commun 2023; 14 (1) : 5317 CENP-E activation by Aurora A and B controls kinetochore fibrous corona disassembly.
Wu J et al. Curr Biol 2023; 33 (5) : 912-925.e6 Microtubule nucleation from the fibrous corona by LIC1-pericentrin promotes chromosome congression.
Li D et al. Theranostics 2021; 11 (11) : 5585-5604 Targeting long noncoding RNA PMIF facilitates osteoprogenitor cells migrating to bone formation surface to promote bone formation during aging.
Application : IHC-P
Reactivity : Mouse
Sudo H et al. Oncotarget 2016; 7 (33) : 53712-53734 The mitotic tensegrity guardian tau protects mammary epithelia from katanin-like1-induced aneuploidy.
Application : ICC/IF
Reactivity : Human
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REVIEW

DYNC1LI1 antibody Cat. No. GTX120114

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-DYNC1LI1 antibody used in Western Blot (WB). GTX120114
Date : Anonymous submitted on 24-Sep-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HaCaT cells
Amount used : 30μg
Blocking : 5% skim milk in PBST, 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169