APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
5-10μg/ml |
Note :
IHC-P
Human Cerebral Cortex shows cytoplasm staining in some of the neuronal cells.
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Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89566-PEP).
Product Note
This antibody is expected to recognise only one of the three reported isoforms (NP_570722.2, isoform a).
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-KIRNVIQGTELKS, from the C Terminus of the protein sequence according to NP_570722.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
dynein axonemal assembly factor 4 , CILD25 , DYX1 , DYX1C1 , DYXC1 , EKN1 , RD
Cellular Localization
Nucleus , Cytoplasm
Background
This gene encodes a tetratricopeptide repeat domain-containing protein. The encoded protein interacts with estrogen receptors and the heat shock proteins, Hsp70 and Hsp90. An homologous protein in rat has been shown to function in neuronal migration in the developing neocortex. A chromosomal translocation involving this gene is associated with a susceptibility to developmental dyslexia. Mutations in this gene are associated with deficits in reading and spelling. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream cell cycle progression 1 (CCPG1) gene. [provided by RefSeq, Mar 2011]
Database
Research Area
DATA IMAGES
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GTX89566 WB Image
WB analysis of COS1 cell lysates untransfected (left lane) and transfected with full length recombinant human DYX1C1 (right lane) using GTX89566 DYX1C1 (Isoform a) antibody, C-term. Dilution : 0.1μg/ml Loading : 30μg protein in RIPA buffer
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GTX89566 IHC-P Image
IHC-P analysis of human cerebral cortex using GTX89566 DYX1C1 (Isoform a) antibody, C-term. Antigen retrieval : citrate buffer pH 6 Dilution : 5μg/ml
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REFERENCE
There are currently no references for DYX1C1 (Isoform a) antibody, C-term (GTX89566). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for DYX1C1 (Isoform a) antibody, C-term (GTX89566). Be the first to share your experience with this product.