GeneTex
United States (US)

DZIP3 antibody [C1C2-2], Internal

GTX124219_40779_WB_18121410_319.jpg
GTX124219_40779_WB_M_Liver_18121410_685.jpg
GTX124219_40779_IHC_18121410_694.jpg

Cat No. GTX124219

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Positive Control

293T , mouse liver

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DZIP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Daz Interacting Zinc Finger Protein 3,Ppp1R66,Uurf2,Hrul138,Dzip3

Cellular Localization

Cytoplasm

Background

E3 Ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Able to specifically bind RNA.

Database

DATA IMAGES
GTX124219_40779_WB_18121410_319.jpg

GTX124219 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
5% SDS PAGE
GTX124219 diluted at 1:1000

GTX124219_40779_WB_M_Liver_18121410_685.jpg

GTX124219 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
5% SDS PAGE
GTX124219 diluted at 1:1000

GTX124219_40779_IHC_18121410_694.jpg

GTX124219 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using DZIP3(GTX124219) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169