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DZIP3 antibody

Anti-DZIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX124220
Anti-DZIP3 antibody used in Western Blot (WB). GTX124220
Anti-DZIP3 antibody used in Western Blot (WB). GTX124220

Cat. No. GTX124220

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all DZIP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Positive Control

293T , mouse kidney

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DZIP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164125

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DAZ interacting zinc finger protein 3 , PPP1R66 , UURF2 , hRUL138

Cellular Localization

Cytoplasm

Background

E3 Ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Able to specifically bind RNA.

Database

Research Area

DATA IMAGES

Anti-DZIP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX124220

GTX124220 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using DZIP3(GTX124220) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DZIP3 antibody used in Western Blot (WB). GTX124220

GTX124220 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
5% SDS PAGE
GTX124220 diluted at 1:1000

Anti-DZIP3 antibody used in Western Blot (WB). GTX124220

GTX124220 WB Image

Sample (50 ug of whole cell lysate)

A: mouse kidney

5% SDS PAGE

GTX124220 diluted at 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169