GeneTex
United States (US)

DZIP3 antibody

GTX124220_40835_IHC_18121410_208.jpg
GTX124220_40835_WB_18121410_257.jpg
GTX124220_40835_WB_M_Kidney_18121410_403.jpg

Cat No. GTX124220

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Positive Control

293T , mouse kidney
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DZIP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Daz Interacting Zinc Finger Protein 3,Ppp1R66,Uurf2,Hrul138,Dzip3

Cellular Localization

Cytoplasm

Background

E3 Ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Able to specifically bind RNA.

Database

DATA IMAGES
GTX124220_40835_IHC_18121410_208.jpg

GTX124220 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using DZIP3(GTX124220) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX124220_40835_WB_18121410_257.jpg

GTX124220 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
5% SDS PAGE
GTX124220 diluted at 1:1000

GTX124220_40835_WB_M_Kidney_18121410_403.jpg

GTX124220 WB Image

Sample (50 ug of whole cell lysate)

A: mouse kidney

5% SDS PAGE

GTX124220 diluted at 1:500

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169