Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dab1 antibody [N1N3] (GTX119336) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

DAB1 antibody [N1N3] detects DAB1 protein at cytosol on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
DAB1 antibody [N1N3] (GTX119336) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

DAB1 antibody [N1N3] detects DAB1 protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DAB1 protein stained by DAB1 antibody [N1N3] (GTX119336) diluted at 1:500.
Blue: Hoechst 33342 staining.