GeneTex
United States (US)

Dab1 antibody

Dab1-antibody-GTX16675-WB-1_18121409_672.jpg

Cat No. GTX16675

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Mouse
APPLICATION

Application Note

IP: Use at a concentration of 5 μg/ml. WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 67 kDa). Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

64 kDa. ( Note )

Positive Control

WB: 293 cells transfected with Dab1 HA tag IP: Dab1 Knockout brain p14 lysate and Dab1 Wild Type brain p14 lysate
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.08% Sodium Azide; Constituents: PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recognizes Dab1 of 48 kDa, corresponding to amino acids 399-555 of mouse Dab1 GST fusion protein.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Disabled 1,Ai956902,C630028C02Rik,Scm,Scr,Scrambler,Yot,Dab1

Background

Disruption of Dab1 results in aberrant migration of neurons during development and disorganization of laminar structures throughout the brain. Dab1 is thought to function as an adaptor molecule in signal transduction processes. It contains a protein-protein interaction (PI) similar to the phosphotyrosine binding domain of the Shc oncoprotein, it is phosphorylated by the Src protein tyrosine kinase, and it binds to SH2 binding domains in a phosphotyrosine dependent manner. DAB1 acts downstream of reelin in a signalling pathway.

Database

Research Area

DATA IMAGES
Dab1-antibody-GTX16675-WB-1_18121409_672.jpg

GTX16675 WB Image

GTX16675 at 1g/ml staining mouse DGTX1 (80kDa) in mouse brain extract by Western blot. Lane 1: GTX16674 (N terminal). Lane 2: GTX16675 (C terminal).

Package List Price ($)
$ 319