IP: Use at a concentration of 5 μg/ml. WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 67 kDa). Optimal dilutions/concentrations should be determined by the end user.
WB: 293 cells transfected with Dab1 HA tag IP: Dab1 Knockout brain p14 lysate and Dab1 Wild Type brain p14 lysate
Preservative: 0.08% Sodium Azide; Constituents: PBS
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recognizes Dab1 of 48 kDa, corresponding to amino acids 399-555 of mouse Dab1 GST fusion protein.
Protein G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Disruption of Dab1 results in aberrant migration of neurons during development and disorganization of laminar structures throughout the brain. Dab1 is thought to function as an adaptor molecule in signal transduction processes. It contains a protein-protein interaction (PI) similar to the phosphotyrosine binding domain of the Shc oncoprotein, it is phosphorylated by the Src protein tyrosine kinase, and it binds to SH2 binding domains in a phosphotyrosine dependent manner. DAB1 acts downstream of reelin in a signalling pathway.