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Dact2 antibody

Anti-Dact2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85373
Anti-Dact2 antibody used in Western Blot (WB). GTX85373
Anti-Dact2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85373

Cat. No. GTX85373

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($409)
See all Dact2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , SK-N-SH Cell Lysate(GTX23956)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Dact2 antibody was raised against a 12 amino acid synthetic peptide from near the amino terminus of human DACT2.The immunogen is located within amino acids 150 - 200 of Dact2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dishevelled binding antagonist of beta catenin 2 , C6orf116 , DAPPER2 , DPR2 , bA503C24.7

Background

The Wnt signaling cascade is a conserved process in multicellular animals that plays important roles during development and can contribute to cancer and other diseases. Many members of this pathway are also expressed in the postnatal tissues such as brain. One such protein is Dact2, a member of the Dact protein family that was initially identified through binding to Disheveled (Dvl), a cytoplasmic protein essential to Wnt signaling. Dact2 is most prominent during the development of the thymus kidneys, and salivary gland. Dact2 is thought to play a role distinct from that of Dact1 with Dact2 having a greater impact on a b-catenin-independent process termed planar cell polarity/convergent-extension signaling. Furthermore, Dact2 but not Dact1 can inhibit Nodal signaling by promoting the endocytic degradation of TGF-b receptors. At least two isoforms of Dact2 are known to exist.

Database

Research Area

DATA IMAGES

Anti-Dact2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85373

GTX85373 IHC-P Image

IHC-P analysis of human brain tissue using GTX85373 Dact2 antibody.
Working concentration : 2.5 μg/ml

Anti-Dact2 antibody used in Western Blot (WB). GTX85373

GTX85373 WB Image

WB analysis of SK-N-SH cell lysate using GTX85373 Dact2 antibody.
Working concentration : 1 μg/ml

Anti-Dact2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85373

GTX85373 IHC-P Image

IHC-P analysis of human brain tissue using GTX85373 Dact2 antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409