GeneTex
United States (US)

Daxx antibody [DAXX-01]

GTX29091_DAXX-antibody-DAXX-01__GTX29091-1_19012723_483.jpg
GTX29091_DAXX-antibody-DAXX-01__GTX29091-2_19012723_974.jpg

Cat No. GTX29091

Host Mouse
Clonality Monoclonal
Clone Name DAXX-01
Isotype IgG1
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

ICC: Use at an assay dependant dilution. WB: Use at a dilution of 1/100. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

81 kDa. ( Note )

Positive Control

HeLa whole cell lysate
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment, corresponding to amino acids 558-740 of Human Daxx. Produced in E. coli</>

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Q9UER7, MGC126246, BING2, 1616, MGC126245, DAXX, DAP6, 603186

Background

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx (1,2). Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways (1,2).

Database

Research Area

DATA IMAGES
GTX29091_DAXX-antibody-DAXX-01__GTX29091-1_19012723_483.jpg

GTX29091 ICC/IF Image

Immunofluorescence staining of Daxx in transfected HeLa human cervix carcinoma cell line. Myc Daxx (green) was stained with anti-human Daxx (DAXX-01) (GTX29091), nuclei were stained with DAPI (blue).
1B - HeLa cells were co-transfected with pCDNA3-MycDaxx and pCDNA3-ASK1HA, which led to translocation of Daxx from nucleus to cytoplasm

GTX29091_DAXX-antibody-DAXX-01__GTX29091-2_19012723_974.jpg

GTX29091 ICC/IF Image

Immunofluorescence staining of Daxx in transfected HeLa human cervix carcinoma cell line. Myc Daxx (green) was stained with anti-human Daxx (DAXX-01) (GTX29091), nuclei were stained with DAPI (blue).
1A - nuclear localization of Daxx in HeLa cells transfected with pCDNA3-MycDaxx

Package List Price ($)
$ 319