GeneTex

  • Country / Location Selection

United States (US)

Daxx antibody

Anti-Daxx antibody used in Western Blot (WB). GTX56298
Anti-Daxx antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX56298
Anti-Daxx antibody used in IHC (Paraffin sections) (IHC-P). GTX56298

Cat. No. GTX56298

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all Daxx products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of Daxx. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

death domain associated protein , BING2 , DAP6 , EAP1 , SMIM40

Cellular Localization

Cytoplasm,nucleoplasm,Nucleolus

Background

This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]

Database

Research Area

DATA IMAGES

Anti-Daxx antibody used in Western Blot (WB). GTX56298

GTX56298 WB Image

WB analysis of HeLa (A), Molt-4 (B), Ramos (C), K562 (D), HEK293T (E) whole cell lysates using GTX56298 Daxx antibody.

Anti-Daxx antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX56298

GTX56298 ICC/IF Image

ICC/IF analysis of formalin-fixed HeLa cells using GTX56298 Daxx antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-Daxx antibody used in IHC (Paraffin sections) (IHC-P). GTX56298

GTX56298 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX56298 Daxx antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

There are currently no references for Daxx antibody (GTX56298). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Daxx antibody (GTX56298). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399