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Dbp-B antibody

Anti-Dbp-B antibody used in Western Blot (WB). GTX48802
Anti-Dbp-B antibody used in Western Blot (WB). GTX48802

Cat. No. GTX48802

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Borrelia burgdorferi
Package
50 μg ($399)
See all Dbp-B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Borrelia burgdorferi

Immunogen

MBP-fusion protein corresponding to Borrelia burgdorferi Dbp-B protein.

Purification

Protein A purified
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

Research Area

DATA IMAGES

Anti-Dbp-B antibody used in Western Blot (WB). GTX48802

GTX48802 WB Image

Western blot showing detection of 0.1 μg of recombinant DbpB protein. Lane 1: Molecular weight markers. Lane 2: MBP-DbpB fusion protein (arrow; expected MW = 60.3 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 μm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4ºC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).

Anti-Dbp-B antibody used in Western Blot (WB). GTX48802

GTX48802 WB Image

WB analysis of various samples using GTX48802 Dbp-B antibody.
Lane 1 : Protein ladder
Lane 2 : MBP-Dbp-B fusion protein
Lane 3 : MBP
Loading : 0.1 μg
Dilution : 1:1000

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399