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Decorin antibody

Anti-Decorin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101250
Anti-Decorin antibody used in IHC (Paraffin sections) (IHC-P). GTX101250
Anti-Decorin antibody used in Western Blot (WB). GTX101250

Cat. No. GTX101250

Reference 7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all Decorin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

A549 , H1299

Predict Reactivity

Mouse, Rabbit, Sheep, Bovine, Dog, Guinea pig, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Decorin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240696

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

decorin , CSCD , DSPG2 , PG40 , PGII , PGS2 , SLRR1B

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to biglycan protein. The encoded protein and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. It contains one attached glycosaminoglycan chain. This protein is capable of suppressing the growth of various tumor cell lines. There are multiple alternatively spliced transcript variants known for this gene. This gene is a candidate gene for Marfan syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Decorin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101250

GTX101250 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using Decorin(GTX101250) antibody at 1:200 dilution.

Anti-Decorin antibody used in IHC (Paraffin sections) (IHC-P). GTX101250

GTX101250 IHC-P Image

Immunohistochemical analysis of paraffin-embedded TOV112D xenograft, using Decorin(GTX101250) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Decorin antibody used in Western Blot (WB). GTX101250

GTX101250 WB Image

Decorin antibody detects DCN protein by western blot analysis.
A. 30 μg A549 whole cell lysate/extract
B. 30 μg H1299 whole cell lysate/extract
10% SDS-PAGE
Decorin antibody (GTX101250) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Ouyang Y et al. Vet Res 2024; 55 (1) : 22 Molecular basis for the different PCV2 susceptibility of T-lymphoblasts in Landrace and Pi?train pigs.
Ayvac? H et al. J Gynecol Obstet Hum Reprod 2021; 50 (10) : 102213 Decorin expression in tubal ectopic and intrauterine pregnancies.
Application : IHC-P
Reactivity : Human
Wong TH et al. Aging (Albany NY) 2020; 13 (1) : 578-597 Decorin inhibits the insulin-like growth factor I signaling in bone marrow mesenchymal stem cells of aged humans.
Application : WB
Reactivity : Rat
Brauchle E et al. Matrix Biol 2018; (Epub) Biomechanical and biomolecular characterization of extracellular matrix structures in human colon carcinomas.
Application : IHC-P
Reactivity : Human
Hinderer S et al. Sci Rep 2018; 8 (1) : 110 Surface functionalization of electrospun scaffolds using recombinant human decorin attracts circulating endothelial progenitor cells.
Application : WB, IP
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169