GeneTex
United States (US)

Decorin antibody

GTX101250_IHC_19011506_170.jpg
GTX101250_39631_IFA_19011506_269.jpg
GTX101250_39631_WB_19011506_866.jpg

Cat No. GTX101250

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

A549 , H1299

Predict Reactivity

Mouse, Rat, Rabbit, Sheep, Bovine, Dog, Guinea pig, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Decorin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Decorin,Cscd,Dspg2,Pg40,Pgii,Pgs2,Slrr1B,Dcn

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to biglycan protein. The encoded protein and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. It contains one attached glycosaminoglycan chain. This protein is capable of suppressing the growth of various tumor cell lines. There are multiple alternatively spliced transcript variants known for this gene. This gene is a candidate gene for Marfan syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101250_IHC_19011506_170.jpg

GTX101250 IHC-P Image

Immunohistochemical analysis of paraffin-embedded TOV112D xenograft, using Decorin(GTX101250) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101250_39631_IFA_19011506_269.jpg

GTX101250 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using Decorin(GTX101250) antibody at 1:200 dilution.

GTX101250_39631_WB_19011506_866.jpg

GTX101250 WB Image

Decorin antibody detects DCN protein by western blot analysis.
A. 30 µg A549 whole cell lysate/extract
B. 30 µg H1299 whole cell lysate/extract
10% SDS-PAGE
Decorin antibody (GTX101250) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169