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Defensin alpha 1 antibody

Anti-Defensin alpha 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64827
Anti-Defensin alpha 1 antibody used in Western Blot (WB). GTX64827
Anti-Defensin alpha 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64827

Cat No. GTX64827

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

10 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-94 of human DEFA1 (NP_004075.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

defensin alpha 1 , DEF1 , DEFA2 , HNP-1 , HP-1 , HP1 , MRS

Cellular Localization

Secreted

Background

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subjec be icopy number variation. [provided by RefSeq, Oc b2014]

Database

Research Area

DATA IMAGES

Anti-Defensin alpha 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64827

GTX64827 IHC-P Image

IHC-P analysis of rat heart tissue using GTX64827 Defensin alpha 1 antibody.
Dilution : 1:100

Anti-Defensin alpha 1 antibody used in Western Blot (WB). GTX64827

GTX64827 WB Image

WB analysis of mouse spleen tissue lysate using GTX64827 Defensin alpha 1 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Defensin alpha 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64827

GTX64827 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX64827 Defensin alpha 1 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339