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Deltex1 antibody [C1C3]

Anti-Deltex1 antibody [C1C3] used in Western Blot (WB). GTX112367
Anti-Deltex1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112367
Anti-Deltex1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX112367

Cat No. GTX112367

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Deltex1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10633307

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

deltex E3 ubiquitin ligase 1 , RNF140 , hDx-1

Cellular Localization

Cytoplasm , Nucleus

Background

Studies in Drosophila have identified this gene as encoding a positive regulator of the Notch-signaling pathway. The human gene encodes a protein of unknown function; however, it may play a role in basic helix-loop-helix transcription factor activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Deltex1 antibody [C1C3] used in Western Blot (WB). GTX112367

GTX112367 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX112367 diluted at 1:1000

Anti-Deltex1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112367

GTX112367 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Deltex1(GTX112367) antibody at 1:200 dilution.

Anti-Deltex1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX112367

GTX112367 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Deltex1(GTX112367) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf