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Dengue virus Capsid protein antibody [GT574]

Anti-Dengue virus Capsid protein antibody [GT574] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633632
Anti-Dengue virus Capsid protein antibody [GT574] used in IHC (Paraffin sections) (IHC-P). GTX633632
Anti-Dengue virus Capsid protein antibody [GT574] used in Western Blot (WB). GTX633632
Anti-Dengue virus Capsid protein antibody [GT574] used in Western Blot (WB). GTX633632
Anti-Dengue virus Capsid protein antibody [GT574] used in Western Blot (WB). GTX633632

Cat. No. GTX633632

Host

Mouse

Clonality

Monoclonal

Clone Name

GT574

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P

Reactivity

Dengue virus 2, Dengue virus 4
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

Dengue 2-infected BHK-21

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus

Immunogen

Full length Dengue virus Capsid recombinant protein. (Dengue virus 2 (strain 16681 PDK 53))

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888356

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Dengue Virus Capsid , DENV Capsid

Research Area

DATA IMAGES

Anti-Dengue virus Capsid protein antibody [GT574] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633632

GTX633632 ICC/IF Image

Dengue virus Capsid protein antibody [GT574] detects Dengue virus Capsid protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and infected A549 cells were fixed in 4% paraformaldehyde at RT for 1 hr.
Green: Dengue virus Capsid protein stained by Dengue virus Capsid protein antibody [GT574] (GTX633632) diluted at 1:510.
Blue: nuclear staining.

Anti-Dengue virus Capsid protein antibody [GT574] used in IHC (Paraffin sections) (IHC-P). GTX633632

GTX633632 IHC-P Image

Dengue virus Capsid protein antibody [GT574] detects Dengue virus Capsid protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded BHK-21 infected with dengue virus.
Green: Dengue virus Capsid protein stained by Dengue virus Capsid protein antibody [GT574] (GTX633632) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Dengue virus Capsid protein antibody [GT574] used in Western Blot (WB). GTX633632

GTX633632 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus Capsid protein antibody [GT574] (GTX633632) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Dengue virus Capsid protein antibody [GT574] used in Western Blot (WB). GTX633632

GTX633632 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus Capsid protein antibody [GT574] (GTX633632) diluted at 1:1000.

Anti-Dengue virus Capsid protein antibody [GT574] used in Western Blot (WB). GTX633632

GTX633632 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus Capsid protein antibody [GT574] (GTX633632) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Dengue virus Capsid protein antibody [GT574] Cat. No. GTX633632

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 09-Aug-2018
Score :
Application Tested : ICC/IF
Sample : A549 cells
Blocking : 5% skim milk in PBST
Fixation : 4%paraformaldehyde, 1Hr
Permeabilization /Antigen retrieval : 0.5% triton-X-100
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169