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Dengue virus Capsid protein antibody

Anti-Dengue virus Capsid protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103343
Anti-Dengue virus Capsid protein antibody used in Western Blot (WB). GTX103343

Cat. No. GTX103343

Reference 17

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Dengue virus 2
Package
100 μl ($399),
25 μl ($169)
See all Dengue virus Capsid protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:1000-1:2000
FACS Assay dependent
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Dengue virus Capsid protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240697

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Dengue Virus Capsid , DENV Capsid

Research Area

DATA IMAGES

Anti-Dengue virus Capsid protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103343

GTX103343 ICC/IF Image

GTX103343 IF Image Immunofluorescence analysis of Denque virus 2-infected BHK-21, using Capside Protein (Dengue virus 2 ) antibody (GTX103343) at 1:2000 dilution.

Anti-Dengue virus Capsid protein antibody used in Western Blot (WB). GTX103343

GTX103343 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus Capsid protein antibody (GTX103343) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wu Y et al. Front Microbiol 2022; 13 : 889693 Autophagy Activation Induces p62-Dependent Autophagic Degradation of Dengue Virus Capsid Protein During Infection.
Shen TJ et al. JCI Insight 2022; 7 (21) Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein-mediated viral translation.
Application : WB
Shwetha Shivaprasad et al. PLoS Pathog 2022; 18 (4) : e1010163 Loquacious modulates flaviviral RNA replication in mosquito cells.
Application : WB
Utomo DIS et al. AMB Express 2022; 12 (1) : 8 Humoral immune response induced with dengue virus-like particles serotypes 1 and 4 produced in silkworm.
Application : WB
Liu B et al. Virol Sin 2020; PTEN Lipid Phosphatase Activity Enhances Dengue Virus Production through Akt/FoxO1/Maf1 Signaling.
Application : WB
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REVIEW

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Application Reference
Wu Y et al. Front Microbiol 2022; 13:889693 Autophagy Activation Induces p62-Dependent Autophagic Degradation of Dengue Virus Capsid Protein During Infection.
Shen TJ et al. JCI Insight 2022; 7 (21) Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein-mediated viral translation.
Application : WB
Shwetha Shivaprasad et al. PLoS Pathog 2022; 18 (4):e1010163 Loquacious modulates flaviviral RNA replication in mosquito cells.
Application : WB
Utomo DIS et al. AMB Express 2022; 12 (1):8 Humoral immune response induced with dengue virus-like particles serotypes 1 and 4 produced in silkworm.
Application : WB
Liu B et al. Virol Sin 2020; PTEN Lipid Phosphatase Activity Enhances Dengue Virus Production through Akt/FoxO1/Maf1 Signaling.
Application : WB
Li LH et al. Antiviral Res 2020; 183:104929 A dengue type 2 reporter virus assay amenable to high-throughput screening.
Application : WB
Butler, M et al. Viruses 2020; 12 (6):E654 Cyclin-Dependent Kinases 8 and 19 Regulate Host Cell Metabolism During Dengue Virus Serotype 2 Infection.
Application : WB
Li M et al. Nat Microbiol 2019; 4 (6):985-995 Identification of antiviral roles for the exon-junction complex and nonsense-mediated decay in flaviviral infection.
Ngo AM et al. Elife 2019; 8 The ER membrane protein complex is required to ensure correct topology and stable expression of flavivirus polyproteins.
Application : WB
Cortese M et al. Cell Rep 2019; 27 (9):2579-2592.e6 Reciprocal Effects of Fibroblast Growth Factor Receptor Signaling on Dengue Virus Replication and Virion Production.
Kao JC et al. PLoS Negl Trop Dis 2018; 12 (8):e0006715 The antiparasitic drug niclosamide inhibits dengue virus infection by interfering with endosomal acidification independent of mTOR.
Application : WB
Juan Fidel Osuna-Ramos et al. bioRxiv 2018; (Epub) Ezetimibe inhibits Dengue virus infection in Huh 7 cells by blocking the cholesterol transporter Niemann–Pick C1–like 1 receptor.
Application : FACS
Perry JW et al. J Virol 2018; (Epub) Functional Analysis of the Dengue Virus Genome Using An Insertional Mutagenesis Screen.
Application : WB
Roth H et al. MBio 2017; 8 (1) Flavivirus Infection Uncouples Translation Suppression from Cellular Stress Responses.
Application : ICC/IF
Reyes-Ruiz JM et al. Virology 2017; 515:74-80 Strand-like structures and the nonstructural proteins 5, 3 and 1 are present in the nucleus of mosquito cells infected with dengue virus.
Application : ICC/IF
Campos RK et al. J Virol 2016; (Epub) RPLP1 and RPLP2 are essential flavivirus host factors that promote early viral protein accumulation.
Application : WB
Angel-Ambrocio AH et al. Virus Res 2015; 198:19572 An embryonic heart cell line is susceptible to dengue virus infection.
Application : ICC/IF, FACS
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169