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Dengue virus Capsid protein antibody

Anti-Dengue virus Capsid protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124247
Anti-Dengue virus Capsid protein antibody used in Western Blot (WB). GTX124247

Cat. No. GTX124247

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Dengue virus
Package
100 μl ($399),
25 μl ($169)
See all Dengue virus Capsid protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:2000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

Dengue virus 2 infect BHK-21

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Full length Dengue virus 2 Capsid Recombinant protein (Dengue virus 2 (strain 16681 PDK 53))

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168188

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Dengue Virus Capsid , DENV Capsid

Research Area

DATA IMAGES

Anti-Dengue virus Capsid protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124247

GTX124247 ICC/IF Image

Capsid Protein (Dengue virus) antibody detects Capsid Protein (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in paraformaldehyde.
Green: Capsid Protein (Dengue virus) protein stained by Capsid Protein (Dengue virus) antibody (GTX124247) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus Capsid protein antibody used in Western Blot (WB). GTX124247

GTX124247 WB Image

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: Japanese encephalitis virus infect BHK-21
15% SDS PAGE
GTX124247 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

H?l?ne Boigard et al. Vaccine 2018; 36 (50) : 7728-7736 Dengue-2 virus-like particle (VLP) based vaccine elicits the highest titers of neutralizing antibodies when produced at reduced temperature.
Application : WB
Reid DW et al. J Virol 2018; (Epub) Dengue virus selectively annexes endoplasmic reticulum-associated translation machinery as a strategy for co-opting host cell protein synthesis.
Application : WB
Hunsawong T et al. PLoS Negl Trop Dis 2015; 9 (9) : e0003958 Immunogenic Properties of a BCG Adjuvanted Chitosan Nanoparticle-Based Dengue Vaccine in Human Dendritic Cells.
Application : ELISA
Soto-Acosta R et al. Antiviral Res 2014; 109C : 132-140 Nordihydroguaiaretic acid (NDGA) inhibits replication and viral morphogenesis of dengue virus.
Application : WB
Reactivity : Dengue virus
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169