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Dengue virus Envelope protein antibody [GT643]

Anti-Dengue virus Envelope protein antibody [GT643] used in Western Blot (WB). GTX629117
Anti-Dengue virus Envelope protein antibody [GT643] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629117
Anti-Dengue virus Envelope protein antibody [GT643] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629117
Anti-Dengue virus Envelope protein antibody [GT643] used in IHC (Paraffin sections) (IHC-P). GTX629117

Cat No. GTX629117

Host Mouse
Clonality Monoclonal
Clone Name GT643
Isotype IgG2b
Application WB, ICC/IF, IHC-P, FACS
Reactivity Dengue virus
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:2000
IHC-P 1:100-1:1000
FACS Assay dependent
Not tested in other applications.

Calculated MW

51-60 kDa. ( Note )

Positive Control

Dengue virus 2 infected BHK21 cells
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Recombinant protein encompassing a sequence within the center region of Dengue virus Envelope protein. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-Dengue virus Envelope protein antibody [GT643] used in Western Blot (WB). GTX629117

GTX629117 WB Image

Envelop protein (Dengue virus 2) antibody [GT643] detects Envelop protein (Dengue virus 2) protein by western blot analysis.
A. 30 µg BHK21 whole cell lysate/extract
B. 30 µg whole cell lysate/extract of Dengue virus type 2 infected BHK21 cells
C. 30 µg whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells
12% SDS-PAGE
Envelop protein (Dengue virus 2) antibody [GT643] (GTX629117) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Dengue virus Envelope protein antibody [GT643] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629117

GTX629117 ICC/IF Image

Envelope protein (Dengue virus) antibody [GT643] detects Envelope protein (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in paraformaldehyde.
Green: Envelope protein (Dengue virus) protein stained by Envelope protein (Dengue virus) antibody [GT643] (GTX629117) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus Envelope protein antibody [GT643] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629117

GTX629117 ICC/IF Image

Envelope protein (Dengue virus) antibody [GT643] detects Envelope protein (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in paraformaldehyde.
Green: Envelope protein (Dengue virus) protein stained by Envelope protein (Dengue virus) antibody [GT643] (GTX629117) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus Envelope protein antibody [GT643] used in IHC (Paraffin sections) (IHC-P). GTX629117

GTX629117 IHC-P Image

Dengue virus Envelope protein antibody [GT643] detects Dengue virus Envelope protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dengue virus BHK-21.
Red: Dengue virus Envelope protein stained by Dengue virus Envelope protein antibody [GT643] (GTX629117) diluted at 1:2000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
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SDS
PBS.pdf