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Dengue virus Envelope protein antibody [GT643]

Anti-Dengue virus Envelope protein antibody [GT643] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629117
Anti-Dengue virus Envelope protein antibody [GT643] used in Western Blot (WB). GTX629117
Anti-Dengue virus Envelope protein antibody [GT643] used in Western Blot (WB). GTX629117
Anti-Dengue virus Envelope protein antibody [GT643] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX629117
Anti-Dengue virus Envelope protein antibody [GT643] used in Western Blot (WB). GTX629117
Anti-Dengue virus Envelope protein antibody [GT643] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629117

Cat. No. GTX629117

Reference 7

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT643

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS, IHC-P (cell pellet)

Reactivity

 Dengue virus 2, Dengue virus 4
Package
100 μl ($399),
25 μl ($169)
See all Dengue virus Envelope protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:2000
FACS Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

Calculated MW

51-60 kDa. ( Note )

Positive Control

Dengue virus type 2 infected BHK21 cells

Product Note

The validated Dengue virus serotype for each application may differ. Please refer to the validated data. This antibody does not cross-react with YFV, JEV, Zika, and CHIKV Envelope protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Recombinant protein encompassing a sequence within the center region of Dengue virus Envelope protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888099

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Dengue Virus Envelope , Dengue Virus E , DENV Envelope , DENV E

Research Area

DATA IMAGES

Anti-Dengue virus Envelope protein antibody [GT643] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629117

GTX629117 ICC/IF Image

Immunofluorescent analysis of Dengue virus infected cells using Dengue virus Envelope protein antibody [GT643] antibody (GTX629117).
Sample: Multiple virus infected cells slide.
Green: Dengue virus Envelope protein antibody [GT643] antibody (GTX629117) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Dengue virus Envelope protein antibody [GT643] used in Western Blot (WB). GTX629117

GTX629117 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus Envelope protein antibody [GT643] (GTX629117) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Dengue virus Envelope protein antibody [GT643] used in Western Blot (WB). GTX629117

GTX629117 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus Envelope protein antibody [GT643] (GTX629117) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Dengue virus Envelope protein antibody [GT643] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX629117

GTX629117 IHC-P (cell pellet) Image

Dengue virus Envelope protein antibody [GT643] detects Dengue virus Envelope protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dengue virus BHK-21.
Red: Dengue virus Envelope protein stained by Dengue virus Envelope protein antibody [GT643] (GTX629117) diluted at 1:2000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Dengue virus Envelope protein antibody [GT643] used in Western Blot (WB). GTX629117

GTX629117 WB Image

Envelop protein (Dengue virus 2) antibody [GT643] detects Envelop protein (Dengue virus 2) protein by western blot analysis.
A. 30 μg BHK21 whole cell lysate/extract
B. 30 μg whole cell lysate/extract of Dengue virus type 2 infected BHK21 cells
C. 30 μg whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells
12% SDS-PAGE
Envelop protein (Dengue virus 2) antibody [GT643] (GTX629117) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Dengue virus Envelope protein antibody [GT643] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629117

GTX629117 ICC/IF Image

Envelope protein (Dengue virus) antibody [GT643] detects Envelope protein (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in paraformaldehyde.
Green: Envelope protein (Dengue virus) protein stained by Envelope protein (Dengue virus) antibody [GT643] (GTX629117) diluted at 1:2000.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Wu KX et al. Acta Pharm Sin B 2023; 13 (5) : 2039-2055 The host-targeting compound peruvoside has a broad-spectrum antiviral activity against positive-sense RNA viruses.
Patthara Kongsuphol et al. Commun Med (Lond) 2021; 1 : 46 A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies.
Kongsuphol P. et al. Communications Medicine 2021; (Epub) A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies.
Kabir MA et al. Sci Rep 2020; 10 (1) : 11906 An antibody panel for highly specific detection and differentiation of Zika virus.
Loe MWC et al. Antiviral Res 2020; 184 : 104954 Betulinic acid exhibits antiviral effects against dengue virus infection.
Application : WB
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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations