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Dengue virus Envelope protein antibody [GT643]

Anti-Dengue virus Envelope protein antibody [GT643] used in Western Blot (WB). GTX629117
Anti-Dengue virus Envelope protein antibody [GT643] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629117
Anti-Dengue virus Envelope protein antibody [GT643] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629117
Anti-Dengue virus Envelope protein antibody [GT643] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX629117

Cat. No. GTX629117

Reference 6

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT643

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS, IHC-P (cell pellet)

Reactivity

 Dengue virus
Package
100 μl ($399),
25 μl ($169)
See all Dengue virus Envelope protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:2000
FACS Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

Calculated MW

51-60 kDa. ( Note )

Positive Control

Dengue virus type 2 infected BHK21 cells

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Recombinant protein encompassing a sequence within the center region of Dengue virus Envelope protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888099

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Dengue Virus Envelope , Dengue Virus E , DENV Envelope , DENV E

Research Area

DATA IMAGES

Anti-Dengue virus Envelope protein antibody [GT643] used in Western Blot (WB). GTX629117

GTX629117 WB Image

Envelop protein (Dengue virus 2) antibody [GT643] detects Envelop protein (Dengue virus 2) protein by western blot analysis.
A. 30 μg BHK21 whole cell lysate/extract
B. 30 μg whole cell lysate/extract of Dengue virus type 2 infected BHK21 cells
C. 30 μg whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells
12% SDS-PAGE
Envelop protein (Dengue virus 2) antibody [GT643] (GTX629117) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Dengue virus Envelope protein antibody [GT643] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629117

GTX629117 ICC/IF Image

Envelope protein (Dengue virus) antibody [GT643] detects Envelope protein (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in paraformaldehyde.
Green: Envelope protein (Dengue virus) protein stained by Envelope protein (Dengue virus) antibody [GT643] (GTX629117) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus Envelope protein antibody [GT643] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629117

GTX629117 ICC/IF Image

Envelope protein (Dengue virus) antibody [GT643] detects Envelope protein (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in paraformaldehyde.
Green: Envelope protein (Dengue virus) protein stained by Envelope protein (Dengue virus) antibody [GT643] (GTX629117) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus Envelope protein antibody [GT643] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX629117

GTX629117 IHC-P (cell pellet) Image

Dengue virus Envelope protein antibody [GT643] detects Dengue virus Envelope protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dengue virus BHK-21.
Red: Dengue virus Envelope protein stained by Dengue virus Envelope protein antibody [GT643] (GTX629117) diluted at 1:2000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Patthara Kongsuphol et al. Commun Med (Lond) 2021; 1 : 46 A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies.
Kongsuphol P. et al. Communications Medicine 2021; (Epub) A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies.
Kabir MA et al. Sci Rep 2020; 10 (1) : 11906 An antibody panel for highly specific detection and differentiation of Zika virus.
Loe MWC et al. Antiviral Res 2020; 184 : 104954 Betulinic acid exhibits antiviral effects against dengue virus infection.
Application : WB
Lee JK et al. Antiviral Res 2019; 171 : 104589 Antiviral activity of ST081006 against the dengue virus.
Application : WB
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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations