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Dengue virus Envelope protein antibody

Orthogonal
Anti-Dengue virus Envelope protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX127277
Anti-Dengue virus Envelope protein antibody used in Western Blot (WB). GTX127277
Anti-Dengue virus Envelope protein antibody used in IHC (Paraffin sections) (IHC-P). GTX127277
Anti-Dengue virus Envelope protein antibody used in Western Blot (WB). GTX127277
Anti-Dengue virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127277
Anti-Dengue virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127277
Anti-Dengue virus Envelope protein antibody used in Western Blot (WB). GTX127277

Cat. No. GTX127277

Reference 34

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA, IHC-P (cell pellet)

Reactivity

 Dengue virus 2
Package
100 μl ($399),
25 μl ($169)
See all Dengue virus Envelope protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:2000
IHC-P 1:100-1:1000
ELISA Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Dengue virus 2 infected BHK-21

Product Note

This antibody was raised against Dengue virus 2 Envelope protein, which may cross react with Envelope protein of Dengue virus 3 but does not recognize with Envelope protein of JEV.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Recombinant protein encompassing a sequence within the center region of Dengue virus Envelope protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163414

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Dengue Virus Envelope , Dengue Virus E , DENV Envelope , DENV E

Research Area

DATA IMAGES

Anti-Dengue virus Envelope protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX127277

GTX127277 IHC-P (cell pellet) Image

Dengue virus Envelope protein antibody detects Dengue virus Envelope protein protein at cytoplasm by immunohistochemical analysis.
Sample: BHK-21 cells mock (left) and infected with Dengue virus (right) FFPE Cell Pellet Block.
Green: Dengue virus Envelope protein stained by Dengue virus Envelope protein antibody (GTX127277) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Dengue virus Envelope protein antibody used in Western Blot (WB). GTX127277

GTX127277 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (5 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus Envelope protein antibody (GTX127277) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus Envelope protein antibody used in IHC (Paraffin sections) (IHC-P). GTX127277

GTX127277 IHC-P Image

Dengue virus Envelope protein antibody detects Dengue virus Envelope protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded BHK-21 infected with dengue virus.
Green: Dengue virus Envelope protein stained by Dengue virus Envelope protein antibody (GTX127277) diluted at 1:2000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Dengue virus Envelope protein antibody used in Western Blot (WB). GTX127277

GTX127277 WB Image

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infected BHK-21
C: JEV infected BHK-21
12% SDS PAGE
GTX127277 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127277

GTX127277 ICC/IF Image

Envelope protein (Dengue virus) antibody detects Envelope protein (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: Envelope protein (Dengue virus) protein stained by Envelope protein (Dengue virus) antibody (GTX127277) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127277

GTX127277 ICC/IF Image

Envelope protein (Dengue virus) antibody detects Envelope protein (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: Envelope protein (Dengue virus) protein stained by Envelope protein (Dengue virus) antibody (GTX127277) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus Envelope protein antibody used in Western Blot (WB). GTX127277

GTX127277 WB Image

The data was published in the journal PLoS Negl Trop Dis in 2014. PMID: 25412261

REFERENCE

Application Reference

Zhou S et al. Virus Res 2023; 338 : 199234 Conessine inhibits enveloped viruses replication through up-regulating cholesterol level.
K Tabata et al. Appl Microbiol Biotechnol 2023; Development of flavivirus subviral particles with low cross-reactivity by mutations of a distinct antigenic domain
X Chen et al. Phytomedicine 2023; 119 : 154977 Liang-Ge-San inhibits dengue virus serotype 2 infection by reducing caveolin1-induced cytoplasmic heat shock protein 70 translocation into the plasma membrane
Application : WB, ICC/IF
C Sri-In et al. Sci Rep 2023; 13 (1) : 9098 34-kDa salivary protein enhances duck Tembusu virus infectivity in the salivary glands of Aedes albopictus by modulating the innate immune response
Application : ICC/IF
Yadav K et al. Microbiol Spectr 2023; 11 (2) : e0250322 Mucin Protein of Aedes aegypti Interacts with Dengue Virus 2 and Influences Viral Infection.
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REVIEW

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Application Reference
Zhou S et al. Virus Res 2023; 338:199234 Conessine inhibits enveloped viruses replication through up-regulating cholesterol level.
K Tabata et al. Appl Microbiol Biotechnol 2023; Development of flavivirus subviral particles with low cross-reactivity by mutations of a distinct antigenic domain
X Chen et al. Phytomedicine 2023; 119:154977 Liang-Ge-San inhibits dengue virus serotype 2 infection by reducing caveolin1-induced cytoplasmic heat shock protein 70 translocation into the plasma membrane
Application : WB, ICC/IF
C Sri-In et al. Sci Rep 2023; 13 (1):9098 34-kDa salivary protein enhances duck Tembusu virus infectivity in the salivary glands of Aedes albopictus by modulating the innate immune response
Application : ICC/IF
Yadav K et al. Microbiol Spectr 2023; 11 (2):e0250322 Mucin Protein of Aedes aegypti Interacts with Dengue Virus 2 and Influences Viral Infection.
Brugier A et al. J Virol 2022; 96 (7):e0196221 RACK1 Associates with RNA-Binding Proteins Vigilin and SERBP1 to Facilitate Dengue Virus Replication.
Application : WB
Wu W et al. Molecules 2022; 27 (23) Acetyl-CoA Carboxylase (ACC) Inhibitor, CP640186, Effectively Inhibited Dengue Virus (DENV) Infection via Regulating ACC Phosphorylation.
Utomo DIS et al. AMB Express 2022; 12 (1):8 Humoral immune response induced with dengue virus-like particles serotypes 1 and 4 produced in silkworm.
Application : ELISA
Patrick T Dolan et al. Elife 2021; 10 Principles of dengue virus evolvability derived from genotype-fitness maps in human and mosquito cells.
Cheang KW et al. PLoS Negl Trop Dis 2021; 15 (8):e0009728 Infecting mosquitoes alters DENV-2 characteristics and enhances hemorrhage-induction potential in Stat1-/- mice.
Application : WB
Aviner R et al. Nature 2021; Cotranslational prolyl hydroxylation is essential for flavivirus biogenesis.
Application : WB
Li D et al. Commun Biol 2021; 4 (1):557 Dengue virus-free defective interfering particles have potent and broad anti-dengue virus activity.
Application : WB
Lin JJ et al. PLoS Pathog 2021; 17 (3):e1009480 Aggressive organ penetration and high vector transmissibility of epidemic dengue virus-2 Cosmopolitan genotype in a transmission mouse model.
Application : WB
Shin H et al. Biosens Bioelectron 2021; 183:113208 A graphene oxide-based fluorescent nanosensor to identify antiviral agents via a drug repurposing screen.
Tammineni ER et al. Virology 2021; 553:81-93 Dantrolene hinders dengue virus-induced upregulation and translocation of calmodulin to cardiac cell nuclei.
Application : WB
Kabir MA et al. Sci Rep 2020; 10 (1):11906 An antibody panel for highly specific detection and differentiation of Zika virus.
Labeau A et al. J Virol 2020; 94 (7) A Genome-Wide CRISPR-Cas9 Screen Identifies the Dolichol-Phosphate Mannose Synthase Complex as a Host Dependency Factor for Dengue Virus Infection.
Chuang FK et al. Antimicrob Agents Chemother 2019; (Epub) Anti-inflammatory compound shows therapeutic safety and efficacy against flavivirus infection.
Application : WB
Rothan HA et al. Antiviral Res 2019; 171:104590 Small molecule grp94 inhibitors block dengue and Zika virus replication.
Utomo DIS et al. Mol Biotechnol 2019; (Epub) Formation of Virus-Like Particles of the Dengue Virus Serotype 2 Expressed in Silkworm Larvae.
Application : WB
Sri-In C et al. Insect Biochem Mol Biol 2019; 111:103181 A salivary protein of Aedes aegypti promotes dengue-2 virus replication and transmission.
Application : WB
Cortese M et al. Cell Rep 2019; 27 (9):2579-2592.e6 Reciprocal Effects of Fibroblast Growth Factor Receptor Signaling on Dengue Virus Replication and Virion Production.
H?l?ne Boigard et al. Vaccine 2018; 36 (50):7728-7736 Dengue-2 virus-like particle (VLP) based vaccine elicits the highest titers of neutralizing antibodies when produced at reduced temperature.
Application : WB
Kao JC et al. PLoS Negl Trop Dis 2018; 12 (8):e0006715 The antiparasitic drug niclosamide inhibits dengue virus infection by interfering with endosomal acidification independent of mTOR.
Application : WB
Reid DW et al. J Virol 2018; (Epub) Dengue virus selectively annexes endoplasmic reticulum-associated translation machinery as a strategy for co-opting host cell protein synthesis.
Application : WB
Puschnik AS et al. Cell Rep 2017; 21 (11):3032-3039 A Small-Molecule Oligosaccharyltransferase Inhibitor with Pan-flaviviral Activity.
Application : WB
Tsai TT et al. Antiviral Res 2017; 145:44-53 Targeting heat shock factor 1 as an antiviral strategy against dengue virus replication in-vitro and in-vivo.
Application : WB
Iswardy E et al. Biosens Bioelectron 2017; 95:174-180 A bead-based immunofluorescence-assay on a microfluidic dielectrophoresis platform for rapid dengue virus detection.
Application : ICC/IF
Soto-Acosta R et al. PLoS Pathog 2017; 13 (4):e1006257 DENV up-regulates the HMG-CoA reductase activity through the impairment of AMPK phosphorylation: A potential antiviral target.
Application : WB
Campos RK et al. J Virol 2016; (Epub) RPLP1 and RPLP2 are essential flavivirus host factors that promote early viral protein accumulation.
Application : WB
Cheng YL et al. Sci Rep 2016; 6:32000 Activation of Nrf2 by the dengue virus causes an increase in CLEC5A, which enhances TNF-α production by mononuclear phagocytes.
Application : WB
Marceau C.D. et al. Nature 2016; 535 (7610):159-63 Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens.
Application : WB, ICC/IF
Cheng YL et al. Mediators Inflamm 2015; 2015 (Epub):274025 Dengue Virus Infection Causes the Activation of Distinct NF-κB Pathways for Inducible Nitric Oxide Synthase and TNF-α Expression in RAW264.7 Cells.
Application : ICC/IF
Tsai TT et al. PLoS Negl Trop Dis 2014; 8 (11):e3320 Antibody-dependent enhancement infection facilitates dengue virus-regulated signaling of IL-10 production in monocytes.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169