GeneTex
United States (US)

Dengue virus Envelope protein antibody

GTX127277_40975_WB_DV_18121410_583.jpg
GTX127277_42137_20150723_WB_Den-2_18121410_886.jpg
GTX127277_40975_IFA_18121410_416.jpg
GTX127277_40975_IFA_2_18121410_605.jpg
GTX127277_40975_20181026_IHC-P-FL_virus_19011519_913.jpg

Cat No. GTX127277

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Dengue virus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:2000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

BHK-Den2 , Dengue virus 2-infected BHK-21
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Recombinant protein encompassing a sequence within the center region of Dengue virus Envelope protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX127277_40975_WB_DV_18121410_583.jpg

GTX127277 WB Image

Sample (20 µg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infected BHK-21
C: JEV infected BHK-21
12% SDS PAGE
GTX127277 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127277_42137_20150723_WB_Den-2_18121410_886.jpg

GTX127277 WB Image

Dengue virus Envelope protein antibody detects Dengue virus Envelope protein protein by western blot analysis. Un-infected (-) and infected (+, Dengue virus 2 infection) BHK-21 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus Envelope protein antibody (GTX127277) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127277_40975_IFA_18121410_416.jpg

GTX127277 ICC/IF Image

Envelope protein (Dengue virus) antibody detects Envelope protein (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: Envelope protein (Dengue virus) protein stained by Envelope protein (Dengue virus) antibody (GTX127277) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX127277_40975_IFA_2_18121410_605.jpg

GTX127277 ICC/IF Image

Envelope protein (Dengue virus) antibody detects Envelope protein (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: Envelope protein (Dengue virus) protein stained by Envelope protein (Dengue virus) antibody (GTX127277) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX127277_40975_20181026_IHC-P-FL_virus_19011519_913.jpg

GTX127277 IHC-P Image

Dengue virus Envelope protein antibody detects Dengue virus Envelope protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded BHK-21 infected with dengue virus.
Green: Dengue virus Envelope protein stained by Dengue virus Envelope protein antibody (GTX127277) diluted at 1:2000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169