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Dengue virus Envelope protein antibody

Anti-Dengue virus Envelope protein antibody used in Western Blot (WB). GTX127277
Anti-Dengue virus Envelope protein antibody used in Western Blot (WB). GTX127277
Anti-Dengue virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127277
Anti-Dengue virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127277
Anti-Dengue virus Envelope protein antibody used in IHC (Paraffin sections) (IHC-P). GTX127277

Cat No. GTX127277

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Dengue virus
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:2000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

BHK-Den2 , Dengue virus 2-infected BHK-21

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Recombinant protein encompassing a sequence within the center region of Dengue virus Envelope protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163414

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Dengue Virus Envelope , Dengue Virus E , DENV Envelope , DENV E

Research Area

DATA IMAGES

Anti-Dengue virus Envelope protein antibody used in Western Blot (WB). GTX127277

GTX127277 WB Image

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infected BHK-21
C: JEV infected BHK-21
12% SDS PAGE
GTX127277 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus Envelope protein antibody used in Western Blot (WB). GTX127277

GTX127277 WB Image

Dengue virus Envelope protein antibody detects Dengue virus Envelope protein protein by western blot analysis. Un-infected (-) and infected (+, Dengue virus 2 infection) BHK-21 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus Envelope protein antibody (GTX127277) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127277

GTX127277 ICC/IF Image

Envelope protein (Dengue virus) antibody detects Envelope protein (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: Envelope protein (Dengue virus) protein stained by Envelope protein (Dengue virus) antibody (GTX127277) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127277

GTX127277 ICC/IF Image

Envelope protein (Dengue virus) antibody detects Envelope protein (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: Envelope protein (Dengue virus) protein stained by Envelope protein (Dengue virus) antibody (GTX127277) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus Envelope protein antibody used in IHC (Paraffin sections) (IHC-P). GTX127277

GTX127277 IHC-P Image

Dengue virus Envelope protein antibody detects Dengue virus Envelope protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded BHK-21 infected with dengue virus.
Green: Dengue virus Envelope protein stained by Dengue virus Envelope protein antibody (GTX127277) diluted at 1:2000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

Dengue virus Envelope protein antibody Cat No. GTX127277

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal PLoS Negl Trop Dis in 2014.PMID: 25412261
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf