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Dengue virus Envelope protein antibody

Anti-Dengue virus Envelope protein antibody used in IHC (Paraffin sections) (IHC-P). GTX127277
Anti-Dengue virus Envelope protein antibody used in Western Blot (WB). GTX127277
Anti-Dengue virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127277
Anti-Dengue virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127277
Anti-Dengue virus Envelope protein antibody used in Western Blot (WB). GTX127277
Anti-Dengue virus Envelope protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX127277
Anti-Dengue virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127277
Anti-Dengue virus Envelope protein antibody used in Western Blot (WB). GTX127277

Cat. No. GTX127277

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA, IHC-P (cell pellet)

Reactivity

Dengue virus 2
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:2000
IHC-P 1:100-1:1000
ELISA Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Dengue virus 2 infected BHK-21

Product Note

This antibody was raised against Dengue virus 2 Envelope protein, which may cross react with Envelope protein of Dengue virus 3 but does not recognize with Envelope protein of JEV.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Recombinant protein encompassing a sequence within the center region of Dengue virus Envelope protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163414

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Dengue Virus Envelope , Dengue Virus E , DENV Envelope , DENV E

Research Area

DATA IMAGES

Anti-Dengue virus Envelope protein antibody used in IHC (Paraffin sections) (IHC-P). GTX127277

GTX127277 IHC-P Image

Dengue virus Envelope protein antibody detects Dengue virus Envelope protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded BHK-21 infected with dengue virus.
Green: Dengue virus Envelope protein stained by Dengue virus Envelope protein antibody (GTX127277) diluted at 1:2000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Dengue virus Envelope protein antibody used in Western Blot (WB). GTX127277

GTX127277 WB Image

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infected BHK-21
C: JEV infected BHK-21
12% SDS PAGE
GTX127277 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127277

GTX127277 ICC/IF Image

Envelope protein (Dengue virus) antibody detects Envelope protein (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: Envelope protein (Dengue virus) protein stained by Envelope protein (Dengue virus) antibody (GTX127277) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127277

GTX127277 ICC/IF Image

Envelope protein (Dengue virus) antibody detects Envelope protein (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: Envelope protein (Dengue virus) protein stained by Envelope protein (Dengue virus) antibody (GTX127277) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus Envelope protein antibody used in Western Blot (WB). GTX127277

GTX127277 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (5 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus Envelope protein antibody (GTX127277) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus Envelope protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX127277

GTX127277 IHC-P (cell pellet) Image

Dengue virus Envelope protein antibody detects Dengue virus Envelope protein protein at cytoplasm by immunohistochemical analysis.
Sample: BHK-21 cells mock (left) and infected with Dengue virus (right) FFPE Cell Pellet Block.
Green: Dengue virus Envelope protein stained by Dengue virus Envelope protein antibody (GTX127277) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Dengue virus Envelope protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127277

GTX127277 ICC/IF Image

Dengue virus Envelope protein antibody detects Dengue virus Envelope protein protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dengue virus Envelope protein stained by Dengue virus Envelope protein antibody (GTX127277) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Dengue virus Envelope protein antibody used in Western Blot (WB). GTX127277

GTX127277 WB Image

The data was published in the journal PLoS Negl Trop Dis in 2014. PMID: 25412261

REFERENCE

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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169