GeneTex
United States (US)

Dengue virus NS1 protein antibody (Biotin)

GTX124280-02_41869_WB_Den2_18121410_529.jpg
GTX124280-02_42795_20170406_WB_Dengue-virus-2_18121410_896.jpg

Cat No. GTX124280-02

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Dengue virus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

Dengue virus 2-infected C6/36 , Dengue virus type 2 infected BHK-21
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Dengue virus NS1 protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Biotin

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX124280-02_41869_WB_Den2_18121410_529.jpg

GTX124280-02 WB Image

NS1(Dengue virus) conjugated biotin antibody detects NS1 protein by western blot analysis. Un-infected (-) and infected (+, Den2 infection for ) BHK-21 whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NS1 conjugaed biotin antibody (GTX224280-02) diluted by 1:2000 and developed with Peroxidase-conjugated Streptavidin.

GTX124280-02_42795_20170406_WB_Dengue-virus-2_18121410_896.jpg

GTX124280-02 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (5 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus NS1 protein antibody (GTX124280-02) diluted at 1:2000. The HRP-conjugated Streptavidin antibody (GTX27239) was used to detect the primary antibody.

SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169