GeneTex

  • Country / Location Selection

United States (US)

Dengue virus NS1 protein antibody [GT4212]

Anti-Dengue virus NS1 protein antibody [GT4212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630556
Anti-Dengue virus NS1 protein antibody [GT4212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630556
Anti-Dengue virus NS1 protein antibody [GT4212] used in Western Blot (WB). GTX630556
Anti-Dengue virus NS1 protein antibody [GT4212] used in Western Blot (WB). GTX630556
Anti-Dengue virus NS1 protein antibody [GT4212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630556

Cat. No. GTX630556

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT4212

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Dengue virus 2, Dengue virus 4
Package
100 μl ($399),
25 μl ($169)
See all Dengue virus NS1 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Product Note

The validated Dengue virus serotype for each application may differ. Please refer to the validated data. This antibody does not cross-react with Zika and CHIKV Envelope protein.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Dengue virus NS1 protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888221

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Dengue Virus Non-structural protein 1 , Dengue Virus NS1 , DENV Non-structural protein 1 , DENV NS1

Research Area

DATA IMAGES

Anti-Dengue virus NS1 protein antibody [GT4212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630556

GTX630556 ICC/IF Image

NS1 (Dengue virus ) antibody [GT4212] detects NS1 (Dengue virus ) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS1 (Dengue virus ) protein stained by NS1 (Dengue virus ) antibody [GT4212] (GTX630556) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS1 protein antibody [GT4212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630556

GTX630556 ICC/IF Image

NS1 (DEN) antibody [GT4212] (GTX630556) mouse mAb. IFA of MeOH/Acetone-fixed DEN2-infected BHK-21 cells (1:1000).
Green: non-structural protein 1 (Dengue virus 2) protein stained by NS1 (Dengue virus ) antibody [GT4212] (GTX630556) diluted at 1:1000.
Blue: DAPI staining.

Anti-Dengue virus NS1 protein antibody [GT4212] used in Western Blot (WB). GTX630556

GTX630556 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus NS1 protein antibody [GT4212] (GTX630556) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Dengue virus NS1 protein antibody [GT4212] used in Western Blot (WB). GTX630556

GTX630556 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus NS1 protein antibody [GT4212] (GTX630556) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Dengue virus NS1 protein antibody [GT4212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630556

GTX630556 ICC/IF Image

Immunofluorescent analysis of Dengue virus infected cells using Dengue virus NS1 protein antibody [GT4212] antibody (GTX630556).
Sample: Multiple virus infected cells slide.
Green: Dengue virus NS1 protein antibody [GT4212] antibody (GTX630556) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

P?aszczyca A et al. PLoS Pathog 2019; 15 (5) : e1007736 A novel interaction between dengue virus nonstructural protein 1 and the NS4A-2K-4B precursor is required for viral RNA replication but not for formation of the membranous replication organelle.
Giraldo MI et al. Virus Res 2018; 246 : 1-11 K48-linked polyubiquitination of dengue virus NS1 protein inhibits its interaction with the viral partner NS4B.
Puschnik AS et al. Cell Rep 2017; 21 (11) : 3032-3039 A Small-Molecule Oligosaccharyltransferase Inhibitor with Pan-flaviviral Activity.
Application : WB
Submit a Reference

REVIEW

There are currently no reviews for Dengue virus NS1 protein antibody [GT4212] (GTX630556). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169