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Dengue virus NS1 protein antibody

Anti-Dengue virus NS1 protein antibody used in Western Blot (WB). GTX124280
Anti-Dengue virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124280
Anti-Dengue virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124280
Anti-Dengue virus NS1 protein antibody used in Western Blot (WB). GTX124280
Anti-Dengue virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124280
Anti-Dengue virus NS1 protein antibody used in Western Blot (WB). GTX124280
Anti-Dengue virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124280
Anti-Dengue virus NS1 protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX124280
Anti-Dengue virus NS1 protein antibody used in IHC (Paraffin sections) (IHC-P). GTX124280

Cat. No. GTX124280

Reference 19

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IP, ELISA, IHC-P (cell pellet)

Reactivity

 Dengue virus
Package
100 μl ($399),
25 μl ($169)
See all Dengue virus NS1 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:2000
IHC-P 1:100-1:1000
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

Dengue virus type 1 infected BHK-21 , Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , Dengue virus type 4 infected BHK-21

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Recombinant protein encompassing a sequence within the center region of Dengue virus NS1 protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171351

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Dengue Virus Non-structural protein 1 , Dengue Virus NS1 , DENV Non-structural protein 1 , DENV NS1

Research Area

DATA IMAGES

Anti-Dengue virus NS1 protein antibody used in Western Blot (WB). GTX124280

GTX124280 WB Image

NS1 (Dengue virus ) antibody detects NS1 (Dengue virus ) protein by Western blot analysis. Un-infected (-) and infected (+, DENV2 infection for ) BHK-21 whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NS1 (Dengue virus ) antibody (GTX124280) diluted by 1:2000.

Anti-Dengue virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124280

GTX124280 ICC/IF Image

NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS1 (Dengue virus) protein stained by NS1 (Dengue virus) antibody (GTX124280) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124280

GTX124280 ICC/IF Image

NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
Green: NS1 (Dengue virus) protein stained by NS1 (Dengue virus) antibody (GTX124280) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS1 protein antibody used in Western Blot (WB). GTX124280

GTX124280 WB Image

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: JEV infect BHK-21
12% SDS PAGE
GTX124280 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124280

GTX124280 ICC/IF Image

NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
Green: NS1 (Dengue virus) protein stained by NS1 (Dengue virus) antibody (GTX124280) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS1 protein antibody used in Western Blot (WB). GTX124280

GTX124280 WB Image

NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein by western blot analysis.
A. 66 μg BHK-21 whole cell extract
B. 66 μg whole cell extract of Dengue virus type 1 infected BHK-21 cells
C. 20 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
D. 10 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
E. 66 μg whole cell extract of Dengue virus type 4 infected BHK-21 cells
10% SDS-PAGE
NS1 (Dengue virus) antibody (GTX124280) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Anti-Dengue virus NS1 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124280

GTX124280 ICC/IF Image

NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS1 (Dengue virus) protein stained by NS1 (Dengue virus) antibody (GTX124280) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS1 protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX124280

GTX124280 IHC-P (cell pellet) Image

Dengue virus NS1 protein antibody detects Dengue virus NS1 protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dengue virus BHK-21.
Green: Dengue virus NS1 protein stained by Dengue virus NS1 protein antibody (GTX124280) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Dengue virus NS1 protein antibody used in IHC (Paraffin sections) (IHC-P). GTX124280

GTX124280 IHC-P Image

Dengue virus NS1 protein antibody detects Dengue virus NS1 protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded BHK-21 infected with dengue virus.
Green: Dengue virus NS1 protein stained by Dengue virus NS1 protein antibody (GTX124280) diluted at 1:2000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Shen TJ et al. JCI Insight 2022; 7 (21) Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein-mediated viral translation.
Jhan MK et al. Cells 2021; 10 (11) Polarization of Type 1 Macrophages Is Associated with the Severity of Viral Encephalitis Caused by Japanese Encephalitis Virus and Dengue Virus.
Application : WB
Lin JJ et al. PLoS Pathog 2021; 17 (3) : e1009480 Aggressive organ penetration and high vector transmissibility of epidemic dengue virus-2 Cosmopolitan genotype in a transmission mouse model.
Application : WB
Shen TJ et al. J Immunol Res 2021; 2021 : 6680913 Antiviral Efficacy of the Anesthetic Propofol against Dengue Virus Infection and Cellular Inflammation.
Application : WB
Shen TJ et al. Front Cell Infect Microbiol 2020; 10 : 557610 CNS Immune Profiling in a Dengue Virus-Infected Immunocompetent Outbred ICR Mice Strain.
Application : WB
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Application Reference
Shen TJ et al. JCI Insight 2022; 7 (21) Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein-mediated viral translation.
Jhan MK et al. Cells 2021; 10 (11) Polarization of Type 1 Macrophages Is Associated with the Severity of Viral Encephalitis Caused by Japanese Encephalitis Virus and Dengue Virus.
Application : WB
Lin JJ et al. PLoS Pathog 2021; 17 (3):e1009480 Aggressive organ penetration and high vector transmissibility of epidemic dengue virus-2 Cosmopolitan genotype in a transmission mouse model.
Application : WB
Shen TJ et al. J Immunol Res 2021; 2021:6680913 Antiviral Efficacy of the Anesthetic Propofol against Dengue Virus Infection and Cellular Inflammation.
Application : WB
Shen TJ et al. Front Cell Infect Microbiol 2020; 10:557610 CNS Immune Profiling in a Dengue Virus-Infected Immunocompetent Outbred ICR Mice Strain.
Application : WB
Hsieh TH et al. Front Cell Infect Microbiol 2020; 10:375 Senescence in Monocytes Facilitates Dengue Virus Infection by Increasing Infectivity.
Application : WB
Rosales Ramirez R et al. J Virol 2019; 93 (4) The Dengue Virus Nonstructural Protein 1 (NS1) Is Secreted from Mosquito Cells in Association with the Intracellular Cholesterol Transporter Chaperone Caveolin Complex.
Kao JC et al. PLoS Negl Trop Dis 2018; 12 (8):e0006715 The antiparasitic drug niclosamide inhibits dengue virus infection by interfering with endosomal acidification independent of mTOR.
Application : WB
Romel Rosales Ramirez et al. bioRxiv 2018; (Epub) The dengue virus non-structural protein 1 (NS1) is secreted from mosquito cells in association with the intracellular cholesterol transporter chaperone caveolin complex.
Application : IP
Reid DW et al. J Virol 2018; (Epub) Dengue virus selectively annexes endoplasmic reticulum-associated translation machinery as a strategy for co-opting host cell protein synthesis.
Application : WB
Hafirassou ML et al. Cell Rep 2017; 21 (13):3900-3913 A Global Interactome Map of the Dengue Virus NS1 Identifies Virus Restriction and Dependency Host Factors.
Application : WB
Ho MR et al. Sci Rep 2017; 7 (1):6910 Blockade of dengue virus infection and viral cytotoxicity in neuronal cells in vitro and in vivo by targeting endocytic pathways.
Application : WB
Sun P et al. J Immunol Methods 2016; (Epub) NK cell degranulation as a marker for measuring antibody-dependent cytotoxicity in neutralizing and non-neutralizing human sera from dengue patients.
Application : FACS
Hsu YL et al. Sci Rep 2016; 6:24530 dengue virus infection induces *-lambda1 to facilitate cell migration.
Reactivity : Dengue virus
Cheng YL et al. Sci Rep 2016; 6:32000 Activation of Nrf2 by the dengue virus causes an increase in CLEC5A, which enhances TNF-α production by mononuclear phagocytes.
Application : WB
Chen HR et al. PLoS Negl Trop Dis 2016; 10 (7):e0004828 Dengue Virus Nonstructural Protein 1 Induces Vascular Leakage through Macrophage Migration Inhibitory Factor and Autophagy.
Application : ELISA
Marceau C.D. et al. Nature 2016; 535 (7610):159-63 Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens.
Application : WB
Reactivity : Dengue virus
Sarathy VV et al. J Gen Virol 2015; 96 (10):3035-48 Characterization of lethal dengue virus type 4 (DENV-4) TVP-376 infection in mice lacking both IFN-α/β and IFN-γ receptors (AG129) and comparison with the DENV-2 AG129 mouse model.
Application : ICC/IF
Cheng YL et al. Mediators Inflamm 2015; 2015 (Epub):274025 Dengue Virus Infection Causes the Activation of Distinct NF-κB Pathways for Inducible Nitric Oxide Synthase and TNF-α Expression in RAW264.7 Cells.
Application : WB
Reactivity : Dengue virus
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169