GeneTex
United States (US)

Dengue virus NS1 protein antibody

GTX124280_41808_WB_DENV2_18121410_298.jpg
GTX124280_40793_IFA_DV_18121410_990.jpg
GTX124280_40793_IFA_2_18121410_514.jpg
GTX124280_40793_IFA_18121410_211.jpg
GTX124280_40793_WB_DV_18121410_808.jpg
GTX124280_40793_IFA_4_18121410_957.jpg
GTX124280_40793_WB_multiple-Dengue-virus_18121410_570.jpg
GTX124280_40793_IFA_3_18121410_757.jpg
GTX124280_40793_20181130_IHC-P-FL_Denguevirus_19011519_472.jpg
GTX124280_40793_20181026_IHC-P-FL_virus_19011519_859.jpg

Cat No. GTX124280

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS, IP, ELISA
Reactivity Dengue virus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:2000
IHC-P 1:100-1:1000
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

BHK-21 , Dengue virus 2 infect BHK-21 , JEV infect BHK-21 , Dengue virus type 2 infected BHK-21 , Dengue virus type 1 infected BHK-21 , Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , Dengue virus type 4 infected BHK-21
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Recombinant protein encompassing a sequence within the center region of Dengue virus NS1 protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX124280_41808_WB_DENV2_18121410_298.jpg

GTX124280 WB Image

NS1 (Dengue virus ) antibody detects NS1 (Dengue virus ) protein by Western blot analysis. Un-infected (-) and infected (+, DENV2 infection for ) BHK-21 whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NS1 (Dengue virus ) antibody (GTX124280) diluted by 1:2000.

GTX124280_40793_IFA_DV_18121410_990.jpg

GTX124280 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS1 (Dengue virus 2)(GTX124280) antibody at 1:2000 dilution.

GTX124280_40793_IFA_2_18121410_514.jpg

GTX124280 ICC/IF Image

NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS1 (Dengue virus) protein stained by NS1 (Dengue virus) antibody (GTX124280) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX124280_40793_IFA_18121410_211.jpg

GTX124280 ICC/IF Image

NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
Green: NS1 (Dengue virus) protein stained by NS1 (Dengue virus) antibody (GTX124280) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX124280_40793_WB_DV_18121410_808.jpg

GTX124280 WB Image

Sample (20 µg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: JEV infect BHK-21
12% SDS PAGE
GTX124280 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124280_40793_IFA_4_18121410_957.jpg

GTX124280 ICC/IF Image

NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
Green: NS1 (Dengue virus) protein stained by NS1 (Dengue virus) antibody (GTX124280) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX124280_40793_WB_multiple-Dengue-virus_18121410_570.jpg

GTX124280 WB Image

NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein by western blot analysis.
A. 66 µg BHK-21 whole cell extract
B. 66 µg whole cell extract of Dengue virus type 1 infected BHK-21 cells
C. 20 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
D. 10 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells
E. 66 µg whole cell extract of Dengue virus type 4 infected BHK-21 cells
10% SDS-PAGE
NS1 (Dengue virus) antibody (GTX124280) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

GTX124280_40793_IFA_3_18121410_757.jpg

GTX124280 ICC/IF Image

NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS1 (Dengue virus) protein stained by NS1 (Dengue virus) antibody (GTX124280) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX124280_40793_20181130_IHC-P-FL_Denguevirus_19011519_472.jpg

GTX124280 IHC-P Image

Dengue virus NS1 protein antibody detects Dengue virus NS1 protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dengue virus BHK-21.
Green: Dengue virus NS1 protein stained by Dengue virus NS1 protein antibody (GTX124280) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX124280_40793_20181026_IHC-P-FL_virus_19011519_859.jpg

GTX124280 IHC-P Image

Dengue virus NS1 protein antibody detects Dengue virus NS1 protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded BHK-21 infected with dengue virus.
Green: Dengue virus NS1 protein stained by Dengue virus NS1 protein antibody (GTX124280) diluted at 1:2000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169