GeneTex
  • Country / Location Selection

United States (US)

Dengue virus NS2B protein antibody

Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
Anti-Dengue virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124246
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
Anti-Dengue virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124246

Cat No. GTX124246

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Dengue virus
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , *Dengue virus 1 infected C6/36 mosquito , *Dengue virus 2 infected Huh-7

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Full length Dengue virus 2 NS2B Recombinant protein

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Dengue Virus Non-structural protein 2B , Dengue Virus NS2B , DENV Non-structural protein 2B , DENV NS2B

Research Area

DATA IMAGES

Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246

GTX124246 WB Image

Non-infected (–) and infected (+) Huh-7 whole cell extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS2B protein antibody (GTX124246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246

GTX124246 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (20 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS2B protein antibody (GTX124246) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124246

GTX124246 ICC/IF Image

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246

GTX124246 WB Image

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: Japanese encephalitis virus infect BHK-21
15% SDS PAGE
GTX124246 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124246

GTX124246 ICC/IF Image

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
Blue: Hoechst 33342 staining.

REFERENCE

REVIEW

Dengue virus NS2B protein antibody Cat No. GTX124246

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 20-Jan-2014
Score :
Application Tested : WB
Sample Species : DENV
Sample : Dengue virus 2 infected Huh-7 cells
Amount used : 10μg
Blocking : 5% skin milk, 25°C, 3Hr
Primary Antibody : 1:3000, 4°C, 16Hr
Lane Description : Lane1:Huh-7 cells, Lane2:Dengue virus 2 infected Huh-7 cells
Date : Anonymous submitted on 21-Nov-2013
Score :
Application Tested : WB
Sample Species : DENV
Sample : Dengue virus 1 infected C6/36 mosquito cells
Amount used : 30μg
Blocking : 5% BSA in 1x TBS, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 16Hr
Lane Description : Lane A:Mock, Lane B:Dengue virus 1 infected C6/36 mosquito cells
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169