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Dengue virus NS2B protein antibody

Orthogonal
Anti-Dengue virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124246
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
Anti-Dengue virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124246

Cat. No. GTX124246

Reference 25
5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Dengue virus, Dengue virus 2
Package
100 μl ($399),
25 μl ($169)
See all Dengue virus NS2B protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

Dengue virus 2 infect BHK-21

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Full length Dengue virus 2 NS2B Recombinant protein (Dengue virus 2 (strain 16681 PDK 53))

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170698

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Dengue Virus Non-structural protein 2B , Dengue Virus NS2B , DENV Non-structural protein 2B , DENV NS2B

Research Area

DATA IMAGES

Anti-Dengue virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124246

GTX124246 ICC/IF Image

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246

GTX124246 WB Image

Non-infected (–) and infected (+) Huh-7 whole cell extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS2B protein antibody (GTX124246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246

GTX124246 WB Image

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: Japanese encephalitis virus infect BHK-21
15% SDS PAGE
GTX124246 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246

GTX124246 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extract (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS2B protein antibody (GTX124246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246

GTX124246 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (20 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS2B protein antibody (GTX124246) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124246

GTX124246 ICC/IF Image

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Udawatte DJ et al. Front Cell Infect Microbiol 2022; 12 : 926036 Dengue virus downregulates TNFR1- and TLR3-stimulated NF-κB activation by targeting RIPK1.
Application : WB
Wei-Chun Chen et al. Viruses 2021; 13 (4) (E)-Guggulsterone Inhibits Dengue Virus Replication by Upregulating Antiviral Interferon Responses through the Induction of Heme Oxygenase-1 Expression.
Application : WB
Jenn-Haung Lai et al. iScience 2021; 24 (6) : 102498 Mitochondrial CMPK2 mediates immunomodulatory and antiviral activities through IFN-dependent and IFN-independent pathways.
Application : WB
Aviner R et al. Nature 2021; Cotranslational prolyl hydroxylation is essential for flavivirus biogenesis.
Application : WB
Lin JJ et al. PLoS Pathog 2021; 17 (3) : e1009480 Aggressive organ penetration and high vector transmissibility of epidemic dengue virus-2 Cosmopolitan genotype in a transmission mouse model.
Application : WB
Show More +
Submit a Reference

REVIEW

Dengue virus NS2B protein antibody Cat. No. GTX124246

Rating
( Average 5 based on 5 users reviews)
Application
Western Blot(WB)
( Average 5 based on 5 users reviews)
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Cell Rep in 2019.PMID: 31067454
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : DENV
Figure Note : The data was peer reviewed and published in the journal Virulence in 2018. PMID: 29338543
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : DENV
Figure Note : The data was peer reviewed and published in the journal Virulence in 2018. PMID: 29338543
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
Date : Anonymous submitted on 20-Jan-2014
Score :
Application Tested : WB
Sample Species : DENV
Sample : Dengue virus 2 infected Huh-7 cells
Amount used : 10μg
Blocking : 5% skin milk, 25°C, 3Hr
Primary Antibody : 1:3000, 4°C, 16Hr
Lane Description : Lane1:Huh-7 cells, Lane2:Dengue virus 2 infected Huh-7 cells
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
Date : Anonymous submitted on 21-Nov-2013
Score :
Application Tested : WB
Sample Species : DENV
Sample : Dengue virus 1 infected C6/36 mosquito cells
Amount used : 30μg
Blocking : 5% BSA in 1x TBS, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 16Hr
Lane Description : Lane A:Mock, Lane B:Dengue virus 1 infected C6/36 mosquito cells
Submit a Review
Application Reference
Udawatte DJ et al. Front Cell Infect Microbiol 2022; 12:926036 Dengue virus downregulates TNFR1- and TLR3-stimulated NF-κB activation by targeting RIPK1.
Application : WB
Wei-Chun Chen et al. Viruses 2021; 13 (4) (E)-Guggulsterone Inhibits Dengue Virus Replication by Upregulating Antiviral Interferon Responses through the Induction of Heme Oxygenase-1 Expression.
Application : WB
Jenn-Haung Lai et al. iScience 2021; 24 (6):102498 Mitochondrial CMPK2 mediates immunomodulatory and antiviral activities through IFN-dependent and IFN-independent pathways.
Application : WB
Aviner R et al. Nature 2021; Cotranslational prolyl hydroxylation is essential for flavivirus biogenesis.
Application : WB
Lin JJ et al. PLoS Pathog 2021; 17 (3):e1009480 Aggressive organ penetration and high vector transmissibility of epidemic dengue virus-2 Cosmopolitan genotype in a transmission mouse model.
Application : WB
Su YC et al. FASEB J 2020; 34 (6):7283-7294 MicroRNA-155 inhibits dengue virus replication by inducing heme oxygenase-1-mediated antiviral interferon responses.
Application : WB
Ngo AM et al. Elife 2019; 8 The ER membrane protein complex is required to ensure correct topology and stable expression of flavivirus polyproteins.
Application : WB
Lin DL et al. Cell Rep 2019; 27 (6):1666-1674.e4 The ER Membrane Protein Complex Promotes Biogenesis of Dengue and Zika Virus Non-structural Multi-pass Transmembrane Proteins to Support Infection.
Application : WB
Li H et al. Neuron 2019; (Epub) Zika Virus Protease Cleavage of Host Protein Septin-2 Mediates Mitotic Defects in Neural Progenitors.
Application : WB
Chen WC et al. Virulence 2018; 9 (1):588-603 Lucidone suppresses dengue viral replication through the induction of heme oxygenase-1.
Lin CK et al. J Infect Dis 2018; (Epub) Prostasin impairs epithelial growth factor receptor activation to suppress dengue virus propagation.
Application : WB
Hélène Boigard et al. Vaccine 2018; 36 (50):7728-7736 Dengue-2 virus-like particle (VLP) based vaccine elicits the highest titers of neutralizing antibodies when produced at reduced temperature.
Application : WB
Lai JH et al. EMBO Rep 2018; (Epub) Infection with the dengue RNA virus activates TLR9 signaling in human dendritic cells.
Application : ICC/IF
Saisawang C et al. PLoS One 2018; 13 (2):e0193133 Glutathionylation of dengue and Zika NS5 proteins affects guanylyltransferase and RNA dependent RNA polymerase activities.
Application : WB
Reid DW et al. J Virol 2018; (Epub) Dengue virus selectively annexes endoplasmic reticulum-associated translation machinery as a strategy for co-opting host cell protein synthesis.
Application : WB
Liu H et al. J Virol 2017; 91 (9) Endoplasmic Reticulum Protein SCAP Inhibits Dengue Virus NS2B3 Protease by Suppressing Its K27-Linked Polyubiquitylation.
Lee JC et al. European Journal of Medicinal Chemistry 2017; 141:282-292 Discovery of novel diarylpyrazolylquinoline derivatives as potent anti-dengue virus agents.
Application : WB
Yu JS et al. Sci Rep 2017; 7:45171 schisandrin a inhibits dengue viral replication via upregulating antiviral * responses through stat signaling pathway.
Application : WB
Lin CK et al. Sci Rep 2017; 7:44701 Cyclooxygenase-2 facilitates dengue virus replication and serves as a potential target for developing antiviral agents.
Application : WB
Yu JS et al. Antiviral Res 2016; 137:49-57 celastrol inhibits dengue virus replication via up-regulating type i * and downstream *-stimulated responses.
Application : WB
Tseng CK et al. Sci Rep 2016; 6:32176 Human heme oxygenase 1 is a potential host cell factor against dengue virus replication.
Application : WB
Lin CK et al. Planta Med 2016; Butyrolactones and Diketopiperazines from Marine Microbes: Inhibition Effects on Dengue Virus Type 2 Replication.
Application : WB
Marceau C.D. et al. Nature 2016; 535 (7610):159-63 Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens.
Application : WB
Reactivity : Dengue virus
Lee JC et al. Antiviral Res 2015; 116:43109 Characterization of the activity of 2'-C-methylcytidine against dengue virus replication.
Application : WB
Reactivity : Dengue virus
Tseng CH et al. Eur J Med Chem 2014; 79C:66-76 Synthesis, antiproliferative and anti-dengue virus evaluations of 2-aroyl-3-arylquinoline derivatives.
Application : WB
Reactivity : Dengue virus
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169