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Dengue virus NS2B protein antibody

Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
Anti-Dengue virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124246
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
Anti-Dengue virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124246
Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246

Cat No. GTX124246

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Dengue virus
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , *Dengue virus 1 infected C6/36 mosquito , *Dengue virus 2 infected Huh-7

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Full length Dengue virus 2 NS2B Recombinant protein (Dengue virus 2 (strain 16681 PDK 53))

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170698

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Dengue Virus Non-structural protein 2B , Dengue Virus NS2B , DENV Non-structural protein 2B , DENV NS2B

Research Area

DATA IMAGES

Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246

GTX124246 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (20 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS2B protein antibody (GTX124246) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124246

GTX124246 ICC/IF Image

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246

GTX124246 WB Image

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: Japanese encephalitis virus infect BHK-21
15% SDS PAGE
GTX124246 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124246

GTX124246 ICC/IF Image

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246

GTX124246 WB Image

Non-infected (–) and infected (+) Huh-7 whole cell extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS2B protein antibody (GTX124246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Dengue virus NS2B protein antibody Cat No. GTX124246

Rating ( Average 5 based on 5 users reviews)
Application
Western Blot(WB) ( Average 5 based on 5 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Figure Note : The data was peer reviewed and published in the journal Cell Rep in 2019.PMID: 31067454
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : DENV
Figure Note : The data was peer reviewed and published in the journal Virulence in 2018.PMID: 29338543
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : DENV
Figure Note : The data was peer reviewed and published in the journal Virulence in 2018.PMID: 29338543
Date : Anonymous submitted on 20-Jan-2014
Score :
Application Tested : WB
Sample Species : DENV
Sample : Dengue virus 2 infected Huh-7 cells
Amount used : 10μg
Blocking : 5% skin milk, 25°C, 3Hr
Primary Antibody : 1:3000, 4°C, 16Hr
Lane Description : Lane1:Huh-7 cells, Lane2:Dengue virus 2 infected Huh-7 cells
Date : Anonymous submitted on 21-Nov-2013
Score :
Application Tested : WB
Sample Species : DENV
Sample : Dengue virus 1 infected C6/36 mosquito cells
Amount used : 30μg
Blocking : 5% BSA in 1x TBS, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 16Hr
Lane Description : Lane A:Mock, Lane B:Dengue virus 1 infected C6/36 mosquito cells
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf