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Dengue virus NS3 protein antibody [GT2811]

Anti-Dengue virus NS3 protein antibody [GT2811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629477
Anti-Dengue virus NS3 protein antibody [GT2811] used in Western Blot (WB). GTX629477
Anti-Dengue virus NS3 protein antibody [GT2811] used in Western Blot (WB). GTX629477
Anti-Dengue virus NS3 protein antibody [GT2811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629477
Anti-Dengue virus NS3 protein antibody [GT2811] used in Western Blot (WB). GTX629477

Cat No. GTX629477

Host Mouse
Clonality Monoclonal
Clone Name GT2811
Isotype IgG1
Application WB, ICC/IF
Reactivity Dengue virus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

Dengue virus 2-infected C6/36 , Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , *Dengue virus 1 infected C6/36 mosquito
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Dengue virus NS3 protein. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
Anti-Dengue virus NS3 protein antibody [GT2811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629477

GTX629477 ICC/IF Image

NS3 (Dengue virus) antibody [GT2811] detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in paraformaldehyde.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody [GT2811] (GTX629477) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus NS3 protein antibody [GT2811] used in Western Blot (WB). GTX629477

GTX629477 WB Image

NS3 (Dengue Virus 2) antibody [GT2811] detects NS3 protein by western blot analysis.
A. 30 µg BHK21 whole cell lysate/extract
B. 30 µg whole cell lysate/extract of Dengue virus 2 infected BHK21 cells
C. 30 µg whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells
7.5% SDS-PAGE
NS3 (Dengue Virus 2) antibody [GT2811] (GTX629477) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Dengue virus NS3 protein antibody [GT2811] used in Western Blot (WB). GTX629477

GTX629477 WB Image

NS3 (Dengue virus) antibody [GT2811] detects NS3 (Dengue virus) protein by western blot analysis.
A. 66 µg BHK-21 whole cell extract
B. 20 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 10 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells
10% SDS-PAGE
NS3 (Dengue virus) antibody [GT2811] (GTX629477) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Anti-Dengue virus NS3 protein antibody [GT2811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629477

GTX629477 ICC/IF Image

NS3 (Dengue virus) antibody [GT2811] detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in paraformaldehyde.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody [GT2811] (GTX629477) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus NS3 protein antibody [GT2811] used in Western Blot (WB). GTX629477

GTX629477 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (15 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody [GT2811] (GTX629477) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Dengue virus NS3 protein antibody [GT2811] Cat No. GTX629477

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 21-Nov-2013
Score :
Application Tested : WB
Sample Species : DENV
Sample : Dengue virus 1 infected C6/36 mosquito cells
Amount used : 30μg
Blocking : 5% BSA in 1x TBS, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, overnightHr
Lane Description : Lane A:Mock, Lane B:Dengue virus 1 infected C6/36 mosquito cells
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169