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Dengue virus NS3 protein antibody [GT2811]

Anti-Dengue virus NS3 protein antibody [GT2811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629477
Anti-Dengue virus NS3 protein antibody [GT2811] used in Western Blot (WB). GTX629477
Anti-Dengue virus NS3 protein antibody [GT2811] used in Western Blot (WB). GTX629477
Anti-Dengue virus NS3 protein antibody [GT2811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629477
Anti-Dengue virus NS3 protein antibody [GT2811] used in Western Blot (WB). GTX629477

Cat No. GTX629477

Host

Mouse

Clonality

Monoclonal

Clone Name

GT2811

Isotype

IgG1

Application

WB, ICC/IF

Reactivity

Dengue virus
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:2000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

Dengue virus 2-infected C6/36 , Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , *Dengue virus 1 infected C6/36 mosquito

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Dengue virus NS3 protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2801283

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Dengue Virus Non-structural protein 3 , Dengue Virus NS3 , DENV Non-structural protein 3 , DENV NS3

Research Area

DATA IMAGES

Anti-Dengue virus NS3 protein antibody [GT2811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629477

GTX629477 ICC/IF Image

NS3 (Dengue virus) antibody [GT2811] detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in paraformaldehyde.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody [GT2811] (GTX629477) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus NS3 protein antibody [GT2811] used in Western Blot (WB). GTX629477

GTX629477 WB Image

NS3 (Dengue Virus 2) antibody [GT2811] detects NS3 protein by western blot analysis.
A. 30 μg BHK21 whole cell lysate/extract
B. 30 μg whole cell lysate/extract of Dengue virus 2 infected BHK21 cells
C. 30 μg whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells
7.5% SDS-PAGE
NS3 (Dengue Virus 2) antibody [GT2811] (GTX629477) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Dengue virus NS3 protein antibody [GT2811] used in Western Blot (WB). GTX629477

GTX629477 WB Image

NS3 (Dengue virus) antibody [GT2811] detects NS3 (Dengue virus) protein by western blot analysis.
A. 66 μg BHK-21 whole cell extract
B. 20 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 10 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
10% SDS-PAGE
NS3 (Dengue virus) antibody [GT2811] (GTX629477) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Anti-Dengue virus NS3 protein antibody [GT2811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629477

GTX629477 ICC/IF Image

NS3 (Dengue virus) antibody [GT2811] detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in paraformaldehyde.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody [GT2811] (GTX629477) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus NS3 protein antibody [GT2811] used in Western Blot (WB). GTX629477

GTX629477 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody [GT2811] (GTX629477) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Dengue virus NS3 protein antibody [GT2811] Cat No. GTX629477

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 04-May-2021
Score :
Application Tested : WB
Sample Species : DENV
Sample : A549 cells
Amount used : 10 μg
Blocking : 5% skim milk in TBST, 27°C, 1Hr
Primary Antibody : 1:5000, 4°C, 12Hr
Lane Description : Non-infected (mock) and infected (infection) A549 whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody (GTX629477) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Date : Anonymous submitted on 21-Nov-2013
Score :
Application Tested : WB
Sample Species : DENV
Sample : Dengue virus 1 infected C6/36 mosquito cells
Amount used : 30μg
Blocking : 5% BSA in 1x TBS, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Lane Description : Lane A:Mock, Lane B:Dengue virus 1 infected C6/36 mosquito cells
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 379
$ 169
Custom Conjugations