Dengue virus NS3 protein antibody [GT2811]

Name: Dengue virus NS3 protein antibody [GT2811]
Brand: GeneTex
SKU: GTX629477
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-Dengue virus NS3 protein antibody [GT2811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629477

Anti-Dengue virus NS3 protein antibody [GT2811] used in Western Blot (WB). GTX629477

Cat. No. GTX629477

Reference 13

Host	Mouse
Clonality	Monoclonal
Clone Name	GT2811
Isotype	IgG1
Application	WB, ICC/IF
Reactivity	Dengue virus
Package	100 μl ($399), 25 μl ($169)

See all Dengue virus NS3 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:2000

Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

Dengue virus 2 infected BHK21 cells , Dengue virus type 3 infected BHK-21 cells , Dengue virus 2-infected C6/36

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Dengue virus NS3 protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2801283

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Dengue Virus Non-structural protein 3 , Dengue Virus NS3 , DENV Non-structural protein 3 , DENV NS3

Research Area

DATA IMAGES

GTX629477 ICC/IF Image

NS3 (Dengue virus) antibody [GT2811] detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in paraformaldehyde.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody [GT2811] (GTX629477) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX629477 WB Image

NS3 (Dengue Virus 2) antibody [GT2811] detects NS3 protein by western blot analysis.
A. 30 μg BHK21 whole cell lysate/extract
B. 30 μg whole cell lysate/extract of Dengue virus 2 infected BHK21 cells
C. 30 μg whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells
7.5% SDS-PAGE
NS3 (Dengue Virus 2) antibody [GT2811] (GTX629477) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629477 WB Image

NS3 (Dengue virus) antibody [GT2811] detects NS3 (Dengue virus) protein by western blot analysis.
A. 66 μg BHK-21 whole cell extract
B. 20 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 10 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
10% SDS-PAGE
NS3 (Dengue virus) antibody [GT2811] (GTX629477) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

GTX629477 ICC/IF Image

NS3 (Dengue virus) antibody [GT2811] detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in paraformaldehyde.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody [GT2811] (GTX629477) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX629477 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody [GT2811] (GTX629477) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Jenn-Haung Lai et al. iScience 2021; 24 (6) : 102498 Mitochondrial CMPK2 mediates immunomodulatory and antiviral activities through IFN-dependent and IFN-independent pathways.

Application : ICC/IF

Mazeaud C et al. Viruses 2021; 13 (10) The Biogenesis of Dengue Virus Replication Organelles Requires the ATPase Activity of Valosin-Containing Protein.

Application : WB

Sarah Goellner et al. STAR Protoc 2020; 1 (3) : 100173 Replication-Independent Generation and Morphological Analysis of Flavivirus Replication Organelles.

Application : WB, ICC/IF

Labeau A et al. J Virol 2020; 94 (7) A Genome-Wide CRISPR-Cas9 Screen Identifies the Dolichol-Phosphate Mannose Synthase Complex as a Host Dependency Factor for Dengue Virus Infection.

Cerikan B et al. Cell Rep 2020; 32 (1) : 107859 A Non-Replicative Role of the 3' Terminal Sequence of the Dengue Virus Genome in Membranous Replication Organelle Formation.

Application : ICC/IF

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REVIEW

Dengue virus NS3 protein antibody [GT2811] Cat. No. GTX629477

Rating

( Average 5 based on 2 users reviews)

Application

Western Blot(WB)

( Average 5 based on 2 users reviews)

Date : Anonymous submitted on 04-May-2021

Score :

Application Tested : WB

Sample Species : DENV

Sample : A549 cells

Amount used : 10 μg

Blocking : 5% skim milk in TBST, 27°C, 1Hr

Primary Antibody : 1:5000, 4°C, 12Hr

Lane Description : Non-infected (mock) and infected (infection) A549 whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody (GTX629477) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.