GeneTex
United States (US)

Dengue virus NS3 protein antibody

GTX124252_40786_IFA_2_18121410_236.jpg
GTX124252_40786_IFA_4_18121410_388.jpg
GTX124252_40786_IFA_DV_18121410_931.jpg
GTX124252_40786_IFA_18121410_849.jpg
GTX124252_40786_WB_DV_18121410_327.jpg
GTX124252_40786_WB_multiple-Dengue-virus_18121410_888.jpg
GTX124252_42508_20160616_WB_Dengue 2_18121410_123.jpg
GTX124252_40786_IFA_3_18121410_974.jpg

Cat No. GTX124252

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS, IHC
Reactivity Dengue virus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:2000
IHC-P Assay dependent
FACS Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

Dengue virus type 1 infected BHK-21 , Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , Dengue virus type 4 infected BHK-21
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Dengue virus NS3 protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX124252_40786_IFA_2_18121410_236.jpg

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX124252_40786_IFA_4_18121410_388.jpg

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX124252_40786_IFA_DV_18121410_931.jpg

GTX124252 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS3 (Dengue virus 2)(GTX124252) antibody at 1:2000 dilution.

GTX124252_40786_IFA_18121410_849.jpg

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX124252_40786_WB_DV_18121410_327.jpg

GTX124252 WB Image

Sample (20 µg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: JEV infect BHK-21
7.5% SDS PAGE
GTX124252 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124252_40786_WB_multiple-Dengue-virus_18121410_888.jpg

GTX124252 WB Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein by western blot analysis.
A. 30 µg BHK-21 whole cell extract
B. 30 µg whole cell extract of Dengue virus type 1 infected BHK-21 cells
C. 30 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
D. 30 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells
E. 30 µg whole cell extract of Dengue virus type 4 infected BHK-21 cells
7.5% SDS-PAGE
NS3 (Dengue virus) antibody (GTX124252) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

GTX124252_42508_20160616_WB_Dengue 2_18121410_123.jpg

GTX124252 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (15 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody (GTX124252) diluted at 1:2000.

GTX124252_40786_IFA_3_18121410_974.jpg

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169