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Dengue virus NS3 protein antibody

Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252

Cat No. GTX124252

Reference 46
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS, IHC
Reactivity Human, Dengue virus
Package
100 μl ($319),
25 μl ($169)
See all Dengue virus NS3 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:2000
IHC-P Assay dependent
FACS Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

Dengue virus type 1 infected BHK-21 , Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , Dengue virus type 4 infected BHK-21
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Dengue virus NS3 protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Research Area

DATA IMAGES
Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252

GTX124252 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS3 (Dengue virus 2)(GTX124252) antibody at 1:2000 dilution.
Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252

GTX124252 WB Image

Sample (20 µg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: JEV infect BHK-21
7.5% SDS PAGE
GTX124252 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252

GTX124252 WB Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein by western blot analysis.
A. 30 µg BHK-21 whole cell extract
B. 30 µg whole cell extract of Dengue virus type 1 infected BHK-21 cells
C. 30 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
D. 30 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells
E. 30 µg whole cell extract of Dengue virus type 4 infected BHK-21 cells
7.5% SDS-PAGE
NS3 (Dengue virus) antibody (GTX124252) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252

GTX124252 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (15 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody (GTX124252) diluted at 1:2000.
Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.
REFERENCE

Application Reference

Rothan HA et al. Antiviral Res 2019; 171 : 104590 Small molecule grp94 inhibitors block dengue and Zika virus replication.
Rafael K. Campos et al. bioRxiv 2019; (Epub) Ribosomal stalk proteins RPLP1 and RPLP2 promote biogenesis of flaviviral and cellular multi-pass transmembrane proteins.
Application : WB
Reactivity : Human
Hammack C et al. J Virol 2019; Zika virus infection induces DNA damage response in human neural progenitors that enhances viral replication.
Application : WB
Ooi YS et al. Nat Microbiol 2019; An RNA-centric dissection of host complexes controlling flavivirus infection.
Application : WB
Nandan S. Gokhale et al. bioRxiv 2019; (Epub) Altered m6A modification of specific cellular transcripts affects Flaviviridae infection
Application : WB
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REVIEW
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Application Reference
Rothan HA et al. Antiviral Res 2019; 171:104590 Small molecule grp94 inhibitors block dengue and Zika virus replication.
Rafael K. Campos et al. bioRxiv 2019; (Epub) Ribosomal stalk proteins RPLP1 and RPLP2 promote biogenesis of flaviviral and cellular multi-pass transmembrane proteins.
Application : WB
Reactivity : Human
Hammack C et al. J Virol 2019; Zika virus infection induces DNA damage response in human neural progenitors that enhances viral replication.
Application : WB
Ooi YS et al. Nat Microbiol 2019; An RNA-centric dissection of host complexes controlling flavivirus infection.
Application : WB
Nandan S. Gokhale et al. bioRxiv 2019; (Epub) Altered m6A modification of specific cellular transcripts affects Flaviviridae infection
Application : WB
Abernathy E et al. PLoS Biol 2019; 17 (1):e2006926 Differential and convergent utilization of autophagy components by positive-strand RNA viruses.
Application : WB
Reactivity : Human
Frakolaki E et al. Cells 2018; 7 (12) The Role of Tissue Oxygen Tension in Dengue Virus Replication.
Application : WB
Reactivity : Human
Arias-Arias JL et al. Am J Trop Med Hyg 2018; 99 (6):1451-1457 Dengue Virus Infection of Primary Human Smooth Muscle Cells.
Application : ICC/IF
Reactivity : Dengue virus
Ramirez L et al. Virus Res 2018; 258:39-49 Dengue virus enters and exits epithelial cells through both apical and basolateral surfaces and perturbs the apical junctional complex.
Application : ICC/IF
Kao JC et al. PLoS Negl Trop Dis 2018; 12 (8):e0006715 The antiparasitic drug niclosamide inhibits dengue virus infection by interfering with endosomal acidification independent of mTOR.
Application : WB
Juan Fidel Osuna-Ramos et al. bioRxiv 2018; (Epub) Ezetimibe inhibits Dengue virus infection in Huh 7 cells by blocking the cholesterol transporter Niemann–Pick C1–like 1 receptor.
Application : WB, FACS
Margot Cervantes-Salazar et al. bioRxiv 2018; (Epub) The Nonstructural Proteins 3 And 5 From Flavivirus Modulate Nuclear-Cytoplasmic Transport And Innate Immune Response Targeting Nuclear Proteins.
Application : WB
Azia S Evans et al. bioRxiv 2018; 2018 (Epub) BPIFB3 facilitates flavivirus infection by controlling RETREG1-dependent reticulophagy.
Application : ICC/IF
Zhang J et al. Cell Host Microbe 2018; 23 (6):819-831.e5 Flaviviruses Exploit the Lipid Droplet Protein AUP1 to Trigger Lipophagy and Drive Virus Production.
Application : WB
Lai YC et al. Antiviral Res 2018; 155:28-38 Minocycline suppresses dengue virus replication by down-regulation of macrophage migration inhibitory factor-induced autophagy.
Application : WB
Sarathy VV et al. Sci Rep 2018; 8 (1):4900 Characterization of a murine model of non-lethal, symptomatic dengue virus infection.
Application : IHC-P
McFadden MJ et al. Viruses 2018; 10 (2) A Fluorescent Cell-Based System for Imaging Zika Virus Infection in Real-Time.
Application : WB
Reid DW et al. J Virol 2018; (Epub) Dengue virus selectively annexes endoplasmic reticulum-associated translation machinery as a strategy for co-opting host cell protein synthesis.
Application : WB
Hishiki T et al. Front Microbiol 2017; 8:1674 Hirsutine, an Indole Alkaloid of Uncaria rhynchophylla, Inhibits Late Step in Dengue Virus Lifecycle.
Reyes-Ruiz JM et al. Virology 2017; 515:74-80 Strand-like structures and the nonstructural proteins 5, 3 and 1 are present in the nucleus of mosquito cells infected with dengue virus.
Application : ICC/IF
Wu X et al. Cell 2017; (Epub) Intrinsic Immunity Shapes Viral Resistance of Stem Cells.
Application : WB
Wan SW et al. J Immunol 2017; (Epub) Therapeutic Effects of Monoclonal Antibody against Dengue Virus NS1 in a STAT1 Knockout Mouse Model of Dengue Infection.
Application : IHC-P
Lai YC et al. Sci Rep 2017; 7 (1):6975 Antibodies Against Modified NS1 Wing Domain Peptide Protect Against Dengue Virus Infection.
Application : IHC-P
Morales L et al. Biomedica 2017; 37:1 Polyclonal antibodies against recombinant dengue virus NS3 protein.
Application : WB, ICC/IF
Soto-Acosta R et al. PLoS Pathog 2017; 13 (4):e1006257 DENV up-regulates the HMG-CoA reductase activity through the impairment of AMPK phosphorylation: A potential antiviral target.
Application : ICC/IF
Bautista-Carbajal P et al. Virology 2017; 501:188-198 The calmodulin antagonist W-7 (N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride) inhibits DENV infection in Huh-7 cells.
Application : WB
Reactivity : Dengue virus
Campos RK et al. J Virol 2016; (Epub) RPLP1 and RPLP2 are essential flavivirus host factors that promote early viral protein accumulation.
Application : WB
Reactivity : Dengue virus
Sun P et al. J Immunol Methods 2016; (Epub) NK cell degranulation as a marker for measuring antibody-dependent cytotoxicity in neutralizing and non-neutralizing human sera from dengue patients.
Application : FACS
Wu YW et al. Sci Rep 2016; 6:32243 Autophagy-associated dengue vesicles promote viral transmission avoiding antibody neutralization.
Application : ICC/IF
Cheng YL et al. Sci Rep 2016; 6:32000 Activation of Nrf2 by the dengue virus causes an increase in CLEC5A, which enhances TNF-α production by mononuclear phagocytes.
Application : WB
Tsai TT et al. Sci Rep 2016; 6:27670 Microglia retard dengue virus-induced acute viral encephalitis.
Application : IHC-P
Morales L et al. Biomedica 2016; 37:1 Anticuerpos policlonales contra recombinantes de la proteina NS3 del virus dengue: especificos y versatiles.
Marceau C.D. et al. Nature 2016; 535 (7610):159-63 Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens.
Application : WB
Reactivity : Dengue virus
Medin CL et al. J Virol Methods 2015; 211:55-62 A plasmid-based reporter system for live cell imaging of dengue virus infected cells.
Application : ICC/IF
Zou J et al. J Virol 2015; 89 (7):3471-83 Mapping the Interactions between the NS4B and NS3 proteins of dengue virus.
Application : WB
Zou J et al. J Virol 2015; 89 (7):3455-70 Characterization of dengue virus NS4A and NS4B protein interaction.
Application : WB, ICC/IF
Yu CY et al. PLoS Pathog 2015; 11 (12):e1005350 Dengue Virus Impairs Mitochondrial Fusion by Cleaving Mitofusins.
Chu YT et al. Immunology 2015; 146 (1):163-72 Mast cell-macrophage dynamics in modulation of dengue virus infection in skin.
Application : IHC
Naik NG et al. J Virol 2015; 89 (13):6746-60 Mutation of Putative N-Glycosylation Sites on Dengue Virus NS4B Decreases RNA Replication.
Application : WB
Reactivity : Dengue virus
Angel-Ambrocio AH et al. Virus Res 2015; 198:19572 An embryonic heart cell line is susceptible to dengue virus infection.
Application : ICC/IF, FACS
Reactivity : Dengue virus
Wan SW et al. PLoS One 2014; 9 (3):e92495 Protection against dengue virus infection in mice by administration of antibodies against modified nonstructural protein 1.
Application : IHC
Reactivity : Dengue virus
Sarathy VV et al. J Virol 2014; (Epub) a lethal murine infection model for dengue virus 3 in ag129 mice deficient in type i and ii * receptors leads to systemic disease.
Application : IHC-P
Reactivity : Dengue virus
Soto-Acosta R et al. Antiviral Res 2014; 109C:132-140 Nordihydroguaiaretic acid (NDGA) inhibits replication and viral morphogenesis of dengue virus.
Application : WB
Reactivity : Dengue virus
St John AL et al. Elife 2013; 2:e00481 Contributions of mast cells and vasoactive products, leukotrienes and chymase, to dengue virus-induced vascular leakage.
Application : ICC/IF
Barratt-Boyes SM et al. Comp Immunol Microbiol Infect Dis 2012; 35 (3):217-8 Comparative immunology, microbiology and infectious diseases. Introduction.
Application : ICC/IF
Reactivity : Dengue virus
Wu CW et al. Patent. 2012; Method and system for monitoring and recording viral infection process and screening for agents that inhibit virus infection
Application : ICC/IF
Reactivity : Dengue virus
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169