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Dengue virus NS3 protein antibody

Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252
Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252
Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252

Cat No. GTX124252

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS, IP, IHC

Reactivity

Dengue virus
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:2000
IHC-P Assay dependent
FACS Assay dependent
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

Dengue virus type 1 infected BHK-21 , Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , Dengue virus type 4 infected BHK-21

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Dengue virus NS3 protein. (Dengue virus 2 (strain 16681 PDK 53)) The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171668

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Dengue Virus Non-structural protein 3 , Dengue Virus NS3 , DENV Non-structural protein 3 , DENV NS3

Research Area

DATA IMAGES

Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252

GTX124252 WB Image

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: JEV infect BHK-21
7.5% SDS PAGE
GTX124252 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252

GTX124252 WB Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 1 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
D. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
E. 30 μg whole cell extract of Dengue virus type 4 infected BHK-21 cells
7.5% SDS-PAGE
NS3 (Dengue virus) antibody (GTX124252) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Anti-Dengue virus NS3 protein antibody used in Western Blot (WB). GTX124252

GTX124252 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody (GTX124252) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS3 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124252

GTX124252 ICC/IF Image

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS3 (Dengue virus) protein stained by NS3 (Dengue virus) antibody (GTX124252) diluted at 1:2000.
Blue: Hoechst 33342 staining.

REFERENCE

REVIEW

Dengue virus NS3 protein antibody Cat No. GTX124252

Rating ( Average 5 based on 10 users reviews)
Application
Western Blot(WB) ( Average 5 based on 9 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 03-May-2021
Score :
Application Tested : WB
Sample Species : DENV
Sample : A549 cells
Amount used : 10 μg
Blocking : 5% skim milk in TBST, 27°C, 1Hr
Primary Antibody : 1:2000, 4°C, 12Hr
Secondary Antibody : GTX213110-01, 1:2000, 27°C, 2Hr
Lane Description : Non-infected (mock) and infected (infection) A549 whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody (GTX124252) diluted at 1:2000.
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29559699
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB,IP
Sample Species : DENV
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2015.PMID: 26717518
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : DENV
Figure Note : The data was peer reviewed and published in the journal Viruses in 2020.PMID: 32121148
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : DENV
Figure Note : The data was peer reviewed and published in the journal Viruses in 2020.PMID: 32121148
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : DENV
Figure Note : The data was peer reviewed and published in the journal Front Cell Infect Microbiol in 2018.PMID: 30186771
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : DENV
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2015.PMID: 26717518
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : DENV
Figure Note : The data was peer reviewed and published in the journal Front Microbiol in 2019.PMID: 31824450
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : DENV
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2015.PMID: 26717518
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : DENV
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2015.PMID: 26717518
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf