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Dengue virus NS4A protein antibody

Anti-Dengue virus NS4A protein antibody used in Western Blot (WB). GTX132069
Anti-Dengue virus NS4A protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132069
Anti-Dengue virus NS4A protein antibody used in IHC (Paraffin sections) (IHC-P). GTX132069

Cat No. GTX132069

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Dengue virus
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

BHK-21
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Full length Dengue virus NS4A recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dengue virus NS4A antibody , Dengue virus NS4A protein antibody

Research Area

DATA IMAGES
Anti-Dengue virus NS4A protein antibody used in Western Blot (WB). GTX132069

GTX132069 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS4A protein antibody (GTX132069) diluted at 1:1000.

Anti-Dengue virus NS4A protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132069

GTX132069 ICC/IF Image

Dengue virus NS4A protein antibody detects Dengue virus NS4A protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and infected A549 cells were fixed in 4% paraformaldehyde at RT for 1 hr.
Green: Dengue virus NS4A protein stained by Dengue virus NS4A protein antibody (GTX132069) diluted at 1:720.
Blue: nuclear staining.

Anti-Dengue virus NS4A protein antibody used in IHC (Paraffin sections) (IHC-P). GTX132069

GTX132069 IHC-P Image

Dengue virus NS4A protein antibody detects Dengue virus NS4A protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded BHK-21 infected with dengue virus.
Green: Dengue virus NS4A protein stained by Dengue virus NS4A protein antibody (GTX132069) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW

Dengue virus NS4A protein antibody Cat No. GTX132069

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 09-Aug-2018
Score :
Application Tested : ICC/IF
Sample Species : DENV
Sample : A549 cells
Blocking : 5% skim milk in PBST
Fixation : 4%paraformaldehyde, 1Hr
Permeabilization /Antigen retrieval : 0.5% triton-X-100
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf