Dengue virus NS5 protein antibody [GT353]

Cat. No. GTX629446

GTX629446 ICC/IF Image

Dengue virus NS5 protein antibody [GT353] detects Dengue virus NS5 protein at nucleus by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in paraformaldehyde.
Green: Dengue virus NS5 protein stained by Dengue virus NS5 protein antibody [GT353] (GTX629446) diluted at 1:2000.
Blue: Hoechst 33342 staining.

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GTX629446 WB Image

Dengue virus NS5 protein antibody [GT353] detects NS5 protein by western blot analysis.
A. 30 μg BHK21 whole cell lysate/extract
B. 30 μg whole cell lysate/extract of Dengue virus 2 infected BHK21 cells
C. 30 μg whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells
10% SDS-PAGE
Dengue virus NS5 protein antibody [GT353] (GTX629446) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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GTX629446 WB Image

The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Dengue virus NS5 protein antibody [GT353] detects Dengue virus NS5 protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
7.5% SDS-PAGE
Dengue virus NS5 protein antibody [GT353] (GTX629446) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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This Dengue virus NS5 protein antibody [GT353] (GTX629446) is a mouse monoclonal antibody validated for detection of Dengue virus NS5 protein by WB, ICC/IF, and IP. Reactive to dengue virus 2, dengue virus 3, and dengue virus 4.

Host

Mouse
Clonality

Monoclonal
Clone Name

GT353
Isotype

IgG2b
Applications

WB ICC/IF IP
Reactivity

Dengue virus 2, Dengue virus 3, Dengue virus 4