Dengue virus NS5 protein antibody [GT361]

Cat. No. GTX629447

GTX629447 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS5 protein antibody [GT361] (GTX629447) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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GTX629447 WB Image

The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Dengue virus NS5 protein antibody [GT361] detects Dengue virus 2 NS5 protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
7.5% SDS-PAGE
Dengue virus NS5 protein antibody [GT361] (GTX629447) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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GTX629447 ICC/IF Image

Dengue virus NS5 protein antibody [GT361] detects Dengue virus 2 NS5 protein protein at nucleus by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in paraformaldehyde.
Green: NS5 (Dengue virus 2) protein stained by Dengue virus NS5 protein antibody [GT361] (GTX629447) diluted at 1:2000.
Blue: Hoechst 33342 staining.

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GTX629447 WB Image

Dengue virus NS5 protein antibody [GT361] detects Dengue virus 2 NS5 protein by western blot analysis.
A. 30 μg BHK21 whole cell lysate/extract
B. 30 μg whole cell lysate/extract of Dengue virus 2 infected BHK21 cells
C. 30 μg whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells
10% SDS-PAGE
Dengue virus NS5 protein antibody [GT361] (GTX629447) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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GTX629447 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS5 protein antibody [GT361] (GTX629447) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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GTX629447 ICC/IF Image

Dengue virus NS5 protein antibody [GT361] detects Dengue virus 2 NS5 protein at nucleus by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in paraformaldehyde.
Green: NS5 (Dengue virus 2) protein stained by Dengue virus NS5 protein antibody [GT361] (GTX629447) diluted at 1:2000.
Blue: Hoechst 33342 staining.

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This Dengue virus NS5 protein antibody [GT361] (GTX629447) is a mouse monoclonal antibody validated for detection of Dengue virus NS5 protein by WB and ICC/IF. Reactivity has been shown in Dengue virus 2, Dengue virus 3, and Dengue virus 4.

Host

Mouse
Clonality

Monoclonal
Clone Name

GT361
Isotype

IgG1
Applications

WB ICC/IF
Reactivity

Dengue virus 2, Dengue virus 3, Dengue virus 4