GeneTex
Dengue virus NS5 protein antibody
Dengue virus NS5 protein antibody detects Dengue virus NS5 protein protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Dengue virus NS5 protein stained by Dengue virus NS5 protein antibody (GTX103350) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus NS5 protein antibody (GTX103350) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GTX103350 IF Image Immunofluorescence analysis of Denque virus 2-infected BHK-21, using Non-structural protein NS5 (Dengue virus 2 ) antibody (GTX103350) at 1:2000 dilution.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.
NS5 (Dengue virus) antibody detects NS5 (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
7.5% SDS-PAGE
NS5 (Dengue virus) antibody (GTX103350) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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HostRabbit
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ClonalityPolyclonal
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IsotypeIgG
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ApplicationsWB ICC/IF
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ReactivityDengue virus 2