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Dengue virus NS5 protein antibody

Anti-Dengue virus NS5 protein antibody used in Western Blot (WB). GTX103350
Anti-Dengue virus NS5 protein antibody used in Western Blot (WB). GTX103350
Anti-Dengue virus NS5 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103350

Cat. No. GTX103350

Reference 15

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Dengue virus
Package
100 μl ($399),
25 μl ($169)
See all Dengue virus NS5 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Positive Control

BHK-21 , Dengue virus type 2 infected BHK-21 cells , Dengue virus type 3 infected BHK-21 cells

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Dengue virus NS5 protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240701

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Dengue Virus Non-structural protein 5 , Dengue Virus NS5 , DENV Non-structural protein 5 , DENV NS5

Research Area

DATA IMAGES

Anti-Dengue virus NS5 protein antibody used in Western Blot (WB). GTX103350

GTX103350 WB Image

Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with (GTX103350) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS5 protein antibody used in Western Blot (WB). GTX103350

GTX103350 WB Image

NS5 (Dengue virus) antibody detects NS5 (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
7.5% SDS-PAGE
NS5 (Dengue virus) antibody (GTX103350) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Anti-Dengue virus NS5 protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103350

GTX103350 ICC/IF Image

GTX103350 IF Image Immunofluorescence analysis of Denque virus 2-infected BHK-21, using Non-structural protein NS5 (Dengue virus 2 ) antibody (GTX103350) at 1:2000 dilution.

REFERENCE

Application Reference

Wu Y et al. Front Microbiol 2022; 13 : 889693 Autophagy Activation Induces p62-Dependent Autophagic Degradation of Dengue Virus Capsid Protein During Infection.
Shwetha Shivaprasad et al. PLoS Pathog 2022; 18 (4) : e1010163 Loquacious modulates flaviviral RNA replication in mosquito cells.
Application : WB
Weng SC et al. Parasit Vectors 2021; 14 (1) : 376 Blood glucose promotes dengue virus infection in the mosquito Aedes aegypti.
Application : WB
Aviner R et al. Nature 2021; Cotranslational prolyl hydroxylation is essential for flavivirus biogenesis.
Application : WB
De Jes?s-Gonz?lez LA et al. Viruses 2020; 12 (6) The Nuclear Pore Complex: A Target for NS3 Protease of Dengue and Zika Viruses.
Application : WB
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Application Reference
Wu Y et al. Front Microbiol 2022; 13:889693 Autophagy Activation Induces p62-Dependent Autophagic Degradation of Dengue Virus Capsid Protein During Infection.
Shwetha Shivaprasad et al. PLoS Pathog 2022; 18 (4):e1010163 Loquacious modulates flaviviral RNA replication in mosquito cells.
Application : WB
Weng SC et al. Parasit Vectors 2021; 14 (1):376 Blood glucose promotes dengue virus infection in the mosquito Aedes aegypti.
Application : WB
Aviner R et al. Nature 2021; Cotranslational prolyl hydroxylation is essential for flavivirus biogenesis.
Application : WB
De Jes?s-Gonz?lez LA et al. Viruses 2020; 12 (6) The Nuclear Pore Complex: A Target for NS3 Protease of Dengue and Zika Viruses.
Application : WB
Zhang J et al. Cell Host Microbe 2018; 23 (6):819-831.e5 Flaviviruses Exploit the Lipid Droplet Protein AUP1 to Trigger Lipophagy and Drive Virus Production.
Application : WB
Roth H et al. MBio 2017; 8 (1) Flavivirus Infection Uncouples Translation Suppression from Cellular Stress Responses.
Application : ICC/IF
Hafirassou ML et al. Cell Rep 2017; 21 (13):3900-3913 A Global Interactome Map of the Dengue Virus NS1 Identifies Virus Restriction and Dependency Host Factors.
Application : WB
Reyes-Ruiz JM et al. Virology 2017; 515:74-80 Strand-like structures and the nonstructural proteins 5, 3 and 1 are present in the nucleus of mosquito cells infected with dengue virus.
Application : ICC/IF
Marceau CD et al. Nature 2016; 535 (7610):159-63 Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens.
Lim SP et al. PLoS Pathog 2016; 12 (8):e1005737 Potent Allosteric Dengue Virus NS5 Polymerase Inhibitors: Mechanism of Action and Resistance Profiling.
Application : ICC/IF
Marceau C.D. et al. Nature 2016; (Epub) Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens.
Application : WB
Reactivity : Dengue virus
Naik NG et al. J Virol 2015; 89 (13):6746-60 Mutation of Putative N-Glycosylation Sites on Dengue Virus NS4B Decreases RNA Replication.
Application : WB
Reactivity : Dengue virus
Fraser JE et al. Methods Mol Biol 2014; 1138:301-28 Investigating Dengue Virus Nonstructural Protein 5 (NS5) Nuclear Import.
Reactivity : Dengue virus
de Wispelaere M et al. J Virol 2013; 87 (13):7367-81 the small molecules azd0530 and * inhibit dengue virus rna replication via fyn kinase.
Application : WB
Reactivity : Dengue virus
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169