GeneTex
United States (US)

Dengue virus NS5 protein antibody

GTX103350_WB_19011506_517.jpg
GTX103350_39624_WB_multiple-Dengue-virus_19011506_654.jpg
GTX103350_IFA_19011506_603.jpg

Cat No. GTX103350

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Dengue virus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Positive Control

Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Dengue virus NS5 protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX103350_WB_19011506_517.jpg

GTX103350 WB Image

Sample(15 µg of cell lysate)
A: BHK21
B: DV infected BHK21
10% SDS PAGE
GTX103350 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103350_39624_WB_multiple-Dengue-virus_19011506_654.jpg

GTX103350 WB Image

NS5 (Dengue virus) antibody detects NS5 (Dengue virus) protein by western blot analysis.
A. 30 µg BHK-21 whole cell extract
B. 30 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 30 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells
7.5% SDS-PAGE
NS5 (Dengue virus) antibody (GTX103350) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

GTX103350_IFA_19011506_603.jpg

GTX103350 ICC/IF Image

GTX103350 IF Image Immunofluorescence analysis of Denque virus 2-infected BHK-21, using Non-structural protein NS5 (Dengue virus 2 ) antibody (GTX103350) at 1:2000 dilution.

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169