Dengue virus NS5 protein antibody

Cat. No. GTX124253

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, FACS
Reactivity	Dengue virus 2
Package	100 μl ($399), 25 μl ($169)

See all Dengue virus NS5 protein products

View product citations for antibody GTX124253 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:2000
FACS	Assay dependent

Not tested in other applications.

Calculated MW

105 kDa. ( Note )

Positive Control

Dengue virus type 2 infected BHK-21 cells , Dengue virus type 3 infected BHK-21 cells

Product Note

This antibody was raised against Dengue virus 2 NS5 protein, which may cross react with NS5 protein of Dengue virus 1/3/4 but does not recognize with NS5 protein of JEV.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Dengue virus NS5 protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169932

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Dengue Virus Non-structural protein 5 , Dengue Virus NS5 , DENV Non-structural protein 5 , DENV NS5

Research Area

DATA IMAGES

GTX124253 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS5 (Dengue virus 2)(GTX124253) antibody at 1:2000 dilution.

GTX124253 ICC/IF Image

NS5 (Dengue virus) antibody detects NS5 (Dengue virus) protein at nucleus by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS5 (Dengue virus) protein stained by NS5 (Dengue virus) antibody (GTX124253) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX124253 WB Image

NS5 (Dengue virus) antibody detects NS5 (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 1 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
D. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
E. 30 μg whole cell extract of Dengue virus type 4 infected BHK-21 cells
7.5% SDS-PAGE
NS5 (Dengue virus) antibody (GTX124253) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

GTX124253 ICC/IF Image

NS5 (Dengue virus) antibody detects NS5 (Dengue virus) protein at nucleus by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS5 (Dengue virus) protein stained by NS5 (Dengue virus) antibody (GTX124253) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX124253 WB Image

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: JEV infect BHK-21
7.5% SDS PAGE
GTX124253 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124253 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS5 protein antibody (GTX124253) diluted at 1:2000.