GeneTex
United States (US)

Dengue virus NS5 protein antibody

GTX124253_40786_IFA_18121410_676.jpg
GTX124253_40786_IFA_DV_18121410_509.jpg
GTX124253_40786_WB_multiple-Dengue-virus_18121410_311.jpg
GTX124253_40786_IFA_2_18121410_991.jpg
GTX124253_40786_WB_DV_18121410_836.jpg
GTX124253_42585_20160818_WB_Den 2_18121410_388.jpg

Cat No. GTX124253

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, FACS
Reactivity Dengue virus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:2000
FACS Assay dependent
Not tested in other applications.

Calculated MW

105 kDa. ( Note )

Positive Control

Dengue virus type 1 infected BHK-21 , Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , Dengue virus type 4 infected BHK-21
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus 2

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Dengue virus NS5 protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX124253_40786_IFA_18121410_676.jpg

GTX124253 ICC/IF Image

NS5 (Dengue virus) antibody detects NS5 (Dengue virus) protein at nucleus by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS5 (Dengue virus) protein stained by NS5 (Dengue virus) antibody (GTX124253) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX124253_40786_IFA_DV_18121410_509.jpg

GTX124253 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS5 (Dengue virus 2)(GTX124253) antibody at 1:2000 dilution.

GTX124253_40786_WB_multiple-Dengue-virus_18121410_311.jpg

GTX124253 WB Image

NS5 (Dengue virus) antibody detects NS5 (Dengue virus) protein by western blot analysis.
A. 30 µg BHK-21 whole cell extract
B. 30 µg whole cell extract of Dengue virus type 1 infected BHK-21 cells
C. 30 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
D. 30 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells
E. 30 µg whole cell extract of Dengue virus type 4 infected BHK-21 cells
7.5% SDS-PAGE
NS5 (Dengue virus) antibody (GTX124253) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

GTX124253_40786_IFA_2_18121410_991.jpg

GTX124253 ICC/IF Image

NS5 (Dengue virus) antibody detects NS5 (Dengue virus) protein at nucleus by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS5 (Dengue virus) protein stained by NS5 (Dengue virus) antibody (GTX124253) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX124253_40786_WB_DV_18121410_836.jpg

GTX124253 WB Image

Sample (20 µg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: JEV infect BHK-21
7.5% SDS PAGE
GTX124253 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124253_42585_20160818_WB_Den 2_18121410_388.jpg

GTX124253 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts (15 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS5 protein antibody (GTX124253) diluted at 1:2000.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169