Dengue virus NS5 protein antibody

Dengue virus NS5 protein antibody detects Dengue virus NS5 protein protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Dengue virus NS5 protein stained by Dengue virus NS5 protein antibody (GTX124253) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Non-infected (–) and infected (+) BHK-21 whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS5 protein antibody (GTX124253) diluted at 1:2000.

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: JEV infect BHK-21
7.5% SDS PAGE
GTX124253 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NS5 (Dengue virus) antibody detects NS5 (Dengue virus) protein at nucleus by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS5 (Dengue virus) protein stained by NS5 (Dengue virus) antibody (GTX124253) diluted at 1:2000.
Blue: Hoechst 33342 staining.

NS5 (Dengue virus) antibody detects NS5 (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 1 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
D. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
E. 30 μg whole cell extract of Dengue virus type 4 infected BHK-21 cells
7.5% SDS-PAGE
NS5 (Dengue virus) antibody (GTX124253) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NS5 (Dengue virus) antibody detects NS5 (Dengue virus) protein at nucleus by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS5 (Dengue virus) protein stained by NS5 (Dengue virus) antibody (GTX124253) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS5 (Dengue virus 2)(GTX124253) antibody at 1:2000 dilution.